期刊文献+

2型糖尿病大鼠周围神经病变模型的建立及相关指标检测 被引量:9

Establishment of type 2 diabetes model in rat with peripheral neuropathy and detection of indicators
下载PDF
导出
摘要 目的建立2型糖尿病(T2DM)大鼠糖尿病周围神经病变模型,观察坐骨神经形态和功能变化。方法雄性SD大鼠44只,分为普通饲料对照(NC)组、高脂饲料对照(HC)组和糖尿病(DM)组。高脂饲料喂养加小剂量链脲佐菌素(STZ)注射诱导2型糖尿病8周后神经电生理检测坐骨神经动作电位传导速度,电镜检测坐骨神经形态学变化。结果诱导糖尿病8周后,部分大鼠出现神经病变,坐骨神经动作电位传导速度显著降低(P<0.05),并出现明显的脱髓鞘等病理变化。结论高脂饮食结合小剂量STZ注射可复制发病过程近似人类T2DM、伴周围神经病变的大鼠模型,可用于相关病理研究。 Objective To establish a rat model featured peripheral neuropathy associated with type 2 diabetes mellitus(T2DM).Methods 44 male SD rats were divided into normal diet control(NC) group,high fat diet control(HC) group and diabetes mellitus(DM) group.High fat diet plus small dose streptozotocin(STZ) were used to induce T2DM.8 weeks after inducing,nerve conduction velocity was detected by neuroelectrophysiology testing and morphology changes in sciatic nerve were detected by electron microscopy.Results 8 weeks after inducing,part of rats were coincidence with diabetic neuropathy,with lower sciatic nerve conduction velocity(P0.05),and with apparent pathological changes such as demyelination.Conclusion High fat diet combined with small dose STZ could replicate the rat model with progress similar to T2DM of human.The model could be conducive to causing peripheral neuropathy,and could be used to study the pathology mechanisms.
出处 《国际检验医学杂志》 CAS 2013年第1期1-2,5,共3页 International Journal of Laboratory Medicine
关键词 糖尿病 2型 周围神经 神经电生理 显微镜检查 电子 大鼠 diabetes mellitus type 2 peripheral nerves neuroelectrophysiology microscopy electron rats
  • 相关文献

参考文献20

二级参考文献206

共引文献1900

同被引文献106

  • 1赵聪,余剑波.P2X_3受体在神经病理性疼痛中的作用[J].中华临床医师杂志(电子版),2011,5(23):7056-7060. 被引量:2
  • 2杨明华,柴可夫,杨苏蓓.四氧嘧啶致糖尿病大鼠模型血糖稳定性考察[J].中国比较医学杂志,2006,16(8):482-484. 被引量:18
  • 3郑琪蓉,苏青.依帕司他的药理作用及临床应用[J].中国新药与临床杂志,2006,25(11):876-884. 被引量:40
  • 4李红,陈亚青,周筠,贺铭,雷涛,宋利格,张秀珍.醛糖还原酶抑制剂治疗糖尿病周围神经病变的临床研究[J].中国糖尿病杂志,2007,15(11):663-665. 被引量:31
  • 5Ziegler D. Painful diabetic neuropathy:treatment and future as-pects[J].{H}Diabetes/Metabolism Research and Reviews,2008,(Suppl 1):S52-S57.
  • 6Bo C,Xue Z,Yi G. Assessing the quality of reports about randomized controlled trials of acupuncture treatment on Diabetic Peripheral Neuropathy[J].PLoS One,2012,(07):e38461.
  • 7Stevens MJ,Obrosova I,Cao X. Effects of DL-alpha-lipoic acid on peripheral nerve conduction,blood flow,energy metabo-lism,and oxidative stress in experimental diabetic neuropathy[J].{H}DIABETES,2000,(06):1006-1015.
  • 8Premkumar LS,Pabbidi RM. Diabetic peripheral neuropathy:role of reactive oxygen and nitrogen species[J].{H}Cell Biochemistry and Biophysics,2013,(02):373-383.
  • 9Han T,Bai J,Liu W. A systematic review and meta-analysis of α-lipoic acid in the treatment of diabetic peripheral neuropa-thy[J].{H}European Journal of Endocrinology,2012,(04):465-471.
  • 10Storm J,Müller S. Lipoic acid metabolism of Plasmodium-asuitable drug target[J].{H}Current Pharmaceutical Design,2012,(24):3480-3489.

引证文献9

二级引证文献58

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部