摘要
研究了保存时间和保存温度对碳酸盐矿化菌脲酶活性的影响,发现在低温4℃条件下,脲酶活性在29 d内出现不同程度下降;常温短期储存时,酶活性可以保持3 d,3 d后酶活性值下降明显.为提高脲酶的热稳定性,向碳酸盐矿化菌中添加甘油作为保护剂,常温下添加总体积分数20%的甘油,可以提高酶的稳定性,脲酶活性在30℃下3 d内保持不变,但甘油保护作用有限,高温储存超过7 d后酶活性值下降明显;而在保存温度4℃条件下,添加体积分数为10%的甘油,碳酸盐矿化菌可以储存30 d,底物分解量可以达到初始菌液的95%.
Effect of preservation time and temperature on the carbonate-mineralization microbe urease activity is studied. The urease activity decreases in 29 days at 4℃ while it can be kept for 3 days at normal temperature before obvious decrease afterwards. Glycerol is selected as the protective additive to enhance the urease thermostability. The urease activity keep stable for 3 days at 30 ℃, and the best volume fraction for glycerol is 20% at normal temperature. However its effect of preservation is limited, and the urease activity decreases obviously after 7 days at high store temperature. The decomposition ability can reach up to 95% at 4 ℃ after 30 days with 10% glycerol, and the carbonate-mineralization microbe can be kept in reserve for a month.
出处
《东南大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2013年第1期147-151,共5页
Journal of Southeast University：Natural Science Edition
基金
江苏省科技支撑计划资助项目(BK2010062)
关键词
碳酸盐矿化菌
脲酶
甘油
热稳定性
carbonate-mineralization microbe
urease
glycerol
thermostability
