摘要
在厌氧情况下从牛胃里提取的厌氧菌种——肺炎克氏杆菌,在用蔗糖作为诱导物质的条件下,保持一定温度和pH值,在自行研发制造的厌氧流化床生物反应器中,利用甜高粱秸秆厌氧发酵产生氢气。结果表明,该细菌不仅有产氢能力,还能在反应器中吸附的活性炭上形成白色细菌颗粒。当细菌颗粒形成之后,在保持液压恒定的情况下,反应进行3 d之后,细菌会在蔗糖的诱导作用下,利用甜高粱秸秆产生氢气。肺炎克氏杆菌的最佳生长代时为10 h,产氢温度和pH值分别为37℃和6.0。反应过程中液压恒定时间由9.3 h维持14 d之后降低为4.6 h,试验证明,细菌利用甜高粱秸秆阶段的氢气产量明显高于直接利用蔗糖阶段的氢气产量,整个实验过程中氢气含量最高达到28%。
The anaerobic bacteria Klebsiella pneumonia extracted from the cattle stomach in the condi- tion of anaerobic, when used the sucrose as the main induction material in the anaerobic fluidized - bed bioreactor which was invented and manufacted by us, in a certain temperature and pH value, the bacteria could generate hydrogen with the straw which is cellulose anaerobic fermentation. The results showed that the anaerobic bacteria not only was capable of producing hydrogen, but could form white granules onto the adsorption activated carbon in the reactor. After the formation of the granules, in the condition of constant HRT, most sucrose in the fresh medium was consumed after 3 days. Next, theanaerobic bacteria would produce the hydrogen with the sweet sorghum straw in the induction of su- crose. The conclusion was that the Klebsiella pneumonia growing best in 10h, the temperature and the pH value of producing hydrogen were 37~C and 6.0 respectively. During the reaction time, HRT re- duced from 9.3 h to 4. 6h after 14 days. The experiments proved that in the stage of using sweet sor- ghum straw, the hydrogen production was obviously higher than in the stage of using the sugarcane in the medium. The highest content of hydrogen in the whole experiment process could reach up to 28 %.
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第1期140-144,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
河北省科学技术研究与发展计划项目(10393912D)
河北省人力资源与社会保障厅留学回国人员科技活动项目(20101501)
河北省财政专项资助(2011055005)
科技部国际合作项目(2010DFA41230)
第四届中国-南非科技合作联委会项目[国科外字(2009)159号]
关键词
发酵
生物反应器
颗粒
高粱秸秆
产氢
fermentation
bioreactor
granule
sorghum straw
biohydrogen production
