摘要
目的研究中药祛痹镇痛方对胶原诱导性关节炎(collagen induced arthritis,CIA)大鼠滑膜白介素-1β(interleukin-1β,IL-1β)、白介素-8(interleukin-8,IL-8)、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响,探讨祛痹镇痛方治疗CIA的作用机制。方法选用健康雄性Wistar大鼠,随机分为造模组(50只)及正常对照组(正常组,10只),造模组采用牛Ⅱ型胶原(typeⅡcollagen of bovine,BCⅡ)乳剂于大鼠尾根部及颈背部多点注射,建立CIA模型。造模成功后,选取30只造模成功大鼠随机分为模型组(10只)、甲氨喋呤(methotrexate,MTX)组(10只)、祛痹镇痛方组(中药组,10只)。中药组大鼠按22.9g/kg剂量灌胃中药祛痹镇痛方,正常组、模型组灌胃等量生理盐水,每日1次,MTX组按0.78mg/kg剂量灌胃MTX混悬液,每周1次。给药30天后,运用免疫组化法检测大鼠滑膜IL-1β、IL-8、VEGF水平,并予给药前后进行关节炎指数(arthritis index,AI)评分。结果与模型组及本组治疗前比较,治疗后中药组和MTX组大鼠AI评分明显降低(P<0.01)。与正常组比较,模型组大鼠滑膜IL-1β、IL-8、VEGF表达水平明显升高(P<0.01);与模型组比较,中药组和MTX组大鼠滑膜IL-1β、IL-8、VEGF表达水平明显降低(P<0.01),中药组和MTX组组间比较差异无统计学意义(P>0.05)。结论降低CIA大鼠滑膜IL-1β、IL-8、VEGF水平可能是中药祛痹镇痛方治疗CIA的作用机制之一。
Objective To research the effects of Qubi Zhentong Recipe （QZR）on the expressions of interleukin-1β （IL-1 β）, interleukin-8 （IL-8）, and vascular endothelial growth factor （VEGF）in the synovial of rats with collagen-inducing arthritis （CIA）, and to discuss its mechanisms of action. Methods Healthy male Wistar rats were recruited and randomly divided into the model group （ n =50）and the normal control group （ n =10）. Rats of the model group were injected with type II collagen of bovine （BC II ）emulsion in the tail and nape to establish the CIA model. After successful modeling, 30 successfully modeled rats were selected and randomly divided into three groups, i.e., the model group （ n =10）, the QZR group （ n =10） ,and the methotrexate （MTX）group （ n =10）. Rats in the normal control group and the model group were administered with physiological saline by gastrogavage, while those in the QZR group were administered with QZ.R at 22.9 g/kg by gastrogavage. All medication was performed once daily. The rats in the MTX group were administered with MTX suspension at 0.78 mg/kg by gastrogavage, once per week. After 30-day treatment, the levels of IL-1 β, IL-8, and VEGF in the synovial were detected by immunohistochemical method. The arthritis index （AI）was scored before and after medication. Results After treatment the AI score of the QZR group and the MTX group was obviously lower than that of the model group （P 〈0.01）. The AI score of the two drug groups were lower than that before treatment （P 〈 O. 01 ）. Compared with the normal control group, the expression levels of IL-1β, IL-8, and VEGF obviously increased in the model group （ P 〈0.01 ）. Compared with the model group, the expression levels of IL-1β, IL-8, and VEGF were significantly lower in the two drug groups （P 〈0.01 ）. But there was no statistical difference between the QZR group and the MTX group （ P 〉 0.05）. Conclusion Decreasing the expression levels of IL-1β, IL-8, and VEGF in the synovial of CIA rats may be one of the mechanisms for treating CIA by QZ.R.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2013年第1期105-108,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
浙江省中医药科技计划资助项目(No.2010ZB114)
杭州市科技发展计划资助项目(No.20100733Q21)
