摘要
目的探讨联氨基姜黄素(Hydrazinocurcumin,HC)脂质体纳米颗粒(Liposome nanoparticles,NPs)对小鼠乳腺癌4T1细胞增殖、凋亡、侵袭及迁移的影响。方法采用薄膜分散-超声法制备HC-NPs,透射电镜检测纳米颗粒的粒径大小。将4T1细胞分为PBS组、NPs组和HC-NPs组,分别加入10%(v/v)PBS、10%(v/v)NP、10%(v/v)HC-NPs,培养24 h后,MTT法检测细胞的增殖活力;流式细胞术检测细胞周期和凋亡率的变化;刘氏染色法检测细胞的形态;Transwell试验检测4T1细胞侵袭和迁移能力;Western blot检测转导和转录激活因子STAT3及细胞增殖、凋亡、侵袭、迁移相关蛋白的表达水平。结果透射电镜下可见HC-NPs为150 nm左右的脂质体颗粒。HC-NPs对4T1细胞的增殖抑制率明显高于NPs组(P<0.01);与PBS组和NPs组相比,HC-NPs可使细胞形态不规则,诱导细胞周期发生G2/M期阻滞,细胞凋亡率明显增加(P<0.01),明显抑制细胞侵袭和迁移(P<0.01)及STAT3的激活,下调CyclinD1、c-Myc、Bcl-2、Survivin、MMP-9的表达,同时上调Bax的表达(P<0.05)。结论 HC-NPs可能通过抑制STAT3的激活,抑制4T1细胞增殖、侵袭和迁移能力,促进细胞凋亡。
Objective To investigate the effect of liposome nanoparticles of hydrazinocurcumin（HC-NPs） on proliferation,apoptosis,invasion and migration of mouse breast cancer 4T1 cells.Methods Liposome HC-NPs were prepared by film-sonication method,and determined for size by transmission electron microscopy.4T1 cells were divided into three groups and treated with 10%（v / v） PBS,10%（v / v） NP and 10%（v / v） HC-NPs for 24 h respectively,then determined for proliferation activity by MTT method,for cell cycle and apoptosis rate by flow cytometry,for morphological change by Liu staining,for invasion and migration abilities by Transwell test,and for expressions of STAT3 and proteins related to cell proliferation,apoptosis,invasion and migration by Western blot.Results Liposome HC-NPs each at a diameter of about 150 nm were observed under electron microscope.The inhibitory rate of HC-NPs to proliferation of 4T1 cells was significantly higher than that of NPs（P 0.01）.Compared with those in PBS and NPs groups,the morphology of cells in HC-NPs was irregular,while the cell cycle was arrested in G2 / M phase,the apoptosis rate increased significantly（P 0.01）,and the invasion and migration of cells as well as activation of STAT3 were inhibited significantly（P 0.01）.The expressions of CyclinD1,c-Myc,Bcl-2,Survivin,MMP-9 in cells treated with NC-NPs were down-regulated,while that of Bax was up-regulated.Conclusion HC-NPs may promote the apoptosis of 4T1 cells by inhibiting the activation of STAT3 as well as proliferation,invasion and migration of cells.
出处
《中国生物制品学杂志》
CAS
CSCD
2013年第1期76-80,共5页
Chinese Journal of Biologicals
基金
国家自然科学基金(NSFC30971131)
重庆市科委自然科学基金(2009BB5077)
