摘要
目的探讨重组人生长激素(rhGH)对大鼠肠道屏障功能的影响。方法 45只SD大鼠随机均分为3组:A组不予处理;B组肌注生理盐水;C组用肌注rhGH 1.25U.kg-1.d-1治疗。1周后测量各组大鼠小肠绒毛高度、肠腺隐窝深度、黏膜厚度以及绒毛表面积和小肠黏膜CD3+、CD4+、CD8+和IgA+细胞数。结果 C组的小肠绒毛高度、肠腺隐窝深度、黏膜厚度以及绒毛表面积等形态学相关参数明显好于A组和B组(P<0.05);C组小肠黏膜CD3+、CD4+、CD8+和IgA+细胞数高于A组和B组(P<0.05);A组与B组上述指标的差异无统计学意义。结论 rhGH可以增强小肠黏膜屏障功能及免疫屏障功能。
Objective To investigate the effects of recombinant human growth humane（rhGH） on intestinal mucosal barrieral function and immune function in SD rats. Methods Forty-five SD rats were equally randomized into three groups of A（blank control）, B（injected irn. with normal saline for seven days） and C（injected im. with rhGH 1.25U·kg^-1·d^-1 for seven days）. The morphological parameters of small bowel were observed. The cell numbers of CD3＋ , CD4＋ , CD8＋ and IgA＋ in the small bowel mucosal were detected through immunohistochemical technique. Results Compared with groups of A and B, the small bowel mucosal villous height, crypt mucosal depth, mucosal thickness and villous surface area were significantly increased and the cell numbers of CD3＋ , CIM＋ , CD8＋ and IgA＋ in the small bowel mucosal were more in group C（P〈0. 05）, which were not significantly different between group A and group B（P〉0. 05）. Conclusion rhGH may effectively enhance the intestine mechanical barrier function and immune barrier function in SD rats.
出处
《江苏医药》
CAS
北大核心
2013年第1期11-13,共3页
Jiangsu Medical Journal
基金
徐州市科技计划项目(XZZD0803)
关键词
重组人生长激素
肠道屏障
Recombinant human growth humane
Intestinal barrier
