摘要
目的 探讨姜黄素对人结肠癌细胞Lovo体外生长及其细胞周期的影响。方法 MTT法检测肿瘤细胞活性 ,流式细胞仪进行细胞周期时相分析。结果 MTT结果表明姜黄素对Lovo细胞具有细胞毒作用。 2 0 μM、40 μM浓度的姜黄素对细胞的抑制率分别为 41 9%、78.1% ,5～ 10 μM浓度抑制作用较小。姜黄素处理 2 4h ,分裂相细胞的比例可达 8～ 10 %。FCM结果显示姜黄素处理 2 4h时 ,主要使细胞阻滞于S ,G2 +M期 ,使此期的细胞比例增加 ,而G0 / 1期的细胞比例减少。处理 48h后 ,G2 +M期的细胞比例反而降低 ,部分可能由于G2 +M期细胞并发凋亡所致。结论 姜黄素可抑制结肠癌Lovo细胞的生长。
Aim To investigate the effects of curcumin on the growth of human colonic carcinoma cell (Lovo) and the cell cycle change in vitro. Method Activity of tumor cell was detected by MTT and cell cycle phase was analysised by flow cytometry.Result MTT test showed that curcumin was cytotoxic to cells. 20 μM and 40 μM of curcumin caused 41.9% and 78 1% of the proliferative inhibition rate respectively 5 μM and 10μM of curcumin were less effective. Cellular morphology with HE stainning showed that the ratio of cells in mitosis phase was increased after 24 hours treatment with curcumin, MI was up to 8～10%. FCM showed that Lovo cells treated with curcumin were accumulated in S, G 2+M phase in the cell cycle,the ratio of cells in G 0/1 phase was decreased at 24 hours treatment with curcumin. After 48 hours of curcumin treatment, the ratio of cells in G 2+M phase was decreased which might be in part due to apoptosis of cells in G 2+M phase. Conclusion Curcumin could inhibit the growth of Lovo cells in vitro and its mechanisms of action was inferred to arrest the cell cycle in S and G 2+M phase, which prevented cells from entering the next cell cycle.
出处
《胃肠病学和肝病学杂志》
CAS
2000年第2期107-109,共3页
Chinese Journal of Gastroenterology and Hepatology
