摘要
目的探讨环氧化酶(COX)基因-765G/C和-1195G/A多态性与2型糖尿病(T2DM)的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和全自动临床生化分析仪法,对120例T2DM患者和92例健康对照者的COX-2基因多态性和常用临床指标进行检测,比较分析两组间基因型频率和等位基因频率及其临床资料。结果 T2DM组的血糖、餐后2h血糖、TG均高于对照组,差异有统计学意义(P<0.05);而HDL-C、载脂蛋白(Apo)AⅠ均低于对照组,差异有统计学意义(P<0.05);两组-1195G/A位点基因型的频率比较,差异有统计学意义(P<0.05)。结论 COX-2基因-1195G/A多态性可能与皖北地区T2DM的发病相关。
Objective To investigate the relationship between the polymorphism of cyelooxygenase-2（COX-2） gene --765G/C, 1195G/A and type 2 diabetes. Methods Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and fully automatic clinical chemistry analyzer were used to detect COX-2 gene polymorphism and common clinical indicators of 120 ca- ses of T2DM patients and 92 healthy controls. Genotype and allele frequencies and clinical data were comparative analyed between the two groups. Results The blood glucose,postprandial 2 h plasma glucose,TG of the T2DM grout） were higher than the control group, the difference was statistically significant（P〈0.05） ; HDL-C, ApoA I were lower than the control, the difference was statis- tically significant（P〈0.05） ;--1195G/A locus genotype frequency between the two groups was statistically significant（P〈0.05）. Conclusion COX-2 gene --1195G/A polymorphism may be related to the incidence of T2DM in Northern Anhui.
出处
《国际检验医学杂志》
CAS
2012年第21期2569-2571,共3页
International Journal of Laboratory Medicine
关键词
糖尿病
2型
环氧化酶2
基因多态性
diabetes mellitus,type2
cyclooxygenase 2
gene polymorphism
