摘要
目的探讨HBV DNA复制水平与HBV YMDD突变的相关性。方法运用实时荧光定量PCR核酸检测技术,检测150例慢性乙型肝炎(CHB)患者未服用拉米夫定治疗前血清HBV DNA水平以及服用拉米夫定治疗12个月血清中YMDD突变情况。结果治疗前患者血清HBV DNA载量较低者YMDD突变率较低,治疗前病毒载量较高者YMDD突变率也较高(P<0.05)。结论 HBVYMDD突变与治疗前HBV DNA水平呈正相关,治疗前患者血清HBV DNA定量水平检测可作为发生YMDD突变的早期观察指标之一。
Objective To investigate the correlation between the level of HBV DNA replication and HBV YMDD mutation. Methods Using real-time PCR nucleic acid detection technology to detect the serum HBV DNA levels of 150 cases of chronic hepa- titis 13 （CHB） patients before taking lamivudine treatment and after taking lamivudine treatment for 12 months. Results The YM- DD mutation occurrence rate before treatment patients in pctlents with low serum HBV DNA load was lower, and the YMDD muta- lion occurrence rate of the pre-treatment patients with high serum HBV DNA viral load was higher（P〈0.05）. Conclusion The YMDD mutation of HBV could be positively related to pre-treatment HBV DNA levels,and the detection of the pre-treatment ser- um HBV DNA quantitative levels could be used as one of the early indicators of YMDD mutations.
出处
《国际检验医学杂志》
CAS
2012年第22期2724-2725,共2页
International Journal of Laboratory Medicine
