摘要
背景:研究表明微小RNA可通过控制一些重要的转录因子,受体或特异蛋白的表达来调控间充质干细胞的自我更新和分化。目的:探讨微小RNA在间充质干细胞自我更新和分化过程中的调控作用。方法:应用计算机检索2000年1月至2011年11月CNKI数据库相关文章,检索词为"间充质干细胞,miRNA表达差异",并限定文章语言种类为中文。同时计算机检索2000年1月至2011年11月PubMed数据库、Elsevier数据库及Nature数据库相关文章,检索词为"mesenchymal stem cells(MSCs),microRNA(miRNA)",并限定文章语言种类为English。最终纳入符合标准的文献64篇。结果与结论:微小RNA是一种21-25nt的非编码小分子RNA。它可以与靶基因的3’UTR结合在转录水平上调控基因的表达。间充质干细胞是一类可以向骨、软骨、肌肉、神经、脂肪等组织分化的多潜能成体干细胞,其来源广泛,可以从骨髓、脂肪组织、脐带血、脐带等多种组织分离得到。研究表明,微小RNA可通过调控一些特殊的转录因子和发育相关基因的表达来调控间充质干细胞的增殖、自我更新和分化。
BACKGROUND： MicroRNAs （miRNA） play an important role in self-renewal and differentiation of mesenchymal stem cells by modulating expression of some key transcription factors, receptors, and some specific proteins related to the developmental process. OBJECTIVE： To discuss the regulatory roles of miRNAs in self-renewal and differentiation of mesenchymal stem cells. METHODS： PubMed database, Elsevier database and Nature database were retrieved with key words ＂msenchymal stem cells （MSCs）, microRNA （miRNA）＂ for papers published from December 2000 to November 2011 in Chinese and English. A total of 84 papers were retrieved, and 64 of which were included in the final analysis. RESULTS AND CONCLUSION： MicroRNAs are endogenous 21-25nt short non-coding RNAs that can posttranscriptionally modulate gene expression by binding to the 3＇-untranslated region of their target genes. Mesenchymal stem cells can be isolated from bone marrow, adipocyte tissue, cord blood and umbilical cord, and are capable of differentiating into multilineage cell types, such as osteoblasts, chondroblasts, adipocytes, myoblasts and neuroblasts. Recently, studies show that microRNAs play an important role in regulating proliferation, self-renewal and differentiation of mesenchymal stem cells by modulating expression of some specific transcription factors and genes related to the developmental process.
出处
《中国组织工程研究》
CAS
CSCD
2012年第49期9294-9300,共7页
Chinese Journal of Tissue Engineering Research
基金
北京市属市管高等学校人才强教计划资助项目(01500054RA001)~~
