摘要
目的:研究野漆树苷对雷公藤甲素诱导肝损伤的保护作用。方法:采用DMEM培养基培养人系L02肝细胞,加入40μg/ml雷公藤甲素孵育,制备肝细胞损伤模型,继续培养24h后,分别加入75、150、300μg/ml不同剂量的野漆树苷,以及150、300μg/ml不同剂量的阳性对照药谷胱甘肽,继续孵育24h后,采用MTT法测定各组细胞存活率。小鼠随机分为6组,分别每天给予不同剂量药物:野漆树苷高、中、低剂量组(140、70、35mg/kg),阳性对照组给予70mg/kg的甘利欣,模型组及空白对照组分别给予等体积的蒸馏水。6d后,除空白对照组外,其余各组以0.625mg/kg剂量的雷公藤甲素灌胃1次,制备小鼠急性肝损伤模型,18h后眼眶后静脉取血检测血清ALT和AST,测定肝组织匀浆中SOD、MDA、GSH-Px和GST的活性及观察肝脏病理学变化。结果:野漆树苷300、150、75μg/ml剂量组均能显著提高雷公藤甲素损伤的人系L02肝细胞存活率。野漆树苷140、70、35mg/kg剂量组均能显著降低雷公藤甲素所致急性肝损伤小鼠血清ALT和AST活性,降低肝脏MDA含量,增强SOD、GSH-Px和GST活力,并能明显改善肝组织的病理学损伤。结论:野漆树苷对雷公藤甲素诱导的肝损伤具有很好的保护作用。
Study on the protective effect of rhoifolin against hepatic injury induced by triptolide. Method： The protective effects of rhoffolin at the concentration of 75,150,300μg/ml, on the in vitro damage of human L02 liver cells injured by triptolide at the concentration of 40μg/ml were evaluated with cell survival rates, which were determined by MTY assay. Sixty mice were randomly divided into six groups as follows： The drug groups mice were treated with rhoifolin for six day at the dose of 140, 70, 35mg/kg, respectively. The normal control group and the model control group took equivalent amount of distilled water by the same route. And mice in the positive control group were treated with 70mg/kg diammonium glycyrrhizinate. After the last administration, all groups except the normal control were administered with triptolide at dose of 0. 625mg/kg by gavage to induce acute hepatic injury. Thereafter, the contents of ALT, AST in serum, the activities of SOD, MDA, GSH-Px and GST in the liver were measured and the hepatic histological changes were observed by optical microscope. Re- suits： The cell survival rates of human ID2 liver cells injured by triptolide and then treated with rhoifolin at the concentration of 75, 150, 300μg/ml were significantly increased. The contents of ALT, AST in serum were significantly decreased, the content of MDA in the liver was also significantly lowered, and the activities of SOD, MDA, GSH-Px and GST in liver were significantly improved after preventive treatment with rhoifolin at the dose of 140, 70, 35mg/kg. Rhoifolin can ameliorated the hepatic pathological changes. Conclusion： The protective effect of rhoifolin against hepatic injury induced by triptolide was significant in vitro and in vivo.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2012年第6期18-20,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金资助项目(编号:30960495)
