摘要
目的:建立全蝎中游离牛磺酸的含量测定方法。方法:采用50%乙醇超声提取全蝎中的游离氨基酸,异硫氰酸苯酯柱前衍生化后进行反相高效液相色谱分析。色谱柱为Agilent ZORBAX SB-C18(150mm×4.6 mm,5μm),以0.07 mol·L-1醋酸-醋酸钠缓冲液-2.5%乙腈(pH=6.5)为流动相A,乙腈-甲醇-水(40∶15∶45)为流动相B,流速1.0 mL·min-1,检测波长254 nm。结果:牛磺酸在0.008 3～0.414 8μg范围呈良好的线性关系(R2=0.999 9);平均回收率为102.1%,RSD为1.6%(n=6);全蝎中游离牛磺酸含量在0.026 9%～1.27%之间。结论:该方法灵敏度高,重复性好,结果准确可靠,可用于全蝎的质量控制。
Objective： To establish a method for determination of free taurine in Scorpio. Methods： The free taurine in Scorpio extracted with 50% alcohol by ultrasonication was precolumn derived with phenylisothocya- nate. Analysis was performed on an Agilent ZORBAX SB-C18 column （150 mm ×4.6 mm, 5 μm） with gradient e- lution of 0.07 mol· L-1 acetic acid-sodium acetate buffer-2.5% acetonitrile （ pH = 6.5 ） （A） and aeetonitrile- methanol-water （40：15 ： 45 ） （ B ） at the flow rate of 1.0 mL· min - 1, and detected at 254 nm. Results： The linear- ity of the taurine derivative was good in the range of 0. 008 3 -0. 414 8 μg （R2 =0. 999 9）. The average recovery was 102.1% with RSD of 1.6% （n = 6）. The contents of free taurine were from 0. 026 9% to 1.27% in Scorpio. Conclusion： This method shows high sensitivity, repeatability and accuracy, and is proved to be helpful for the quality control of Scorpio effectively.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2013年第2期230-234,共5页
Chinese Journal of New Drugs
基金
2010-2011年度国家药品标准提高科研项目(课题编号:373)
2011年度中国药科大学大学生实践创新训练计划项目
