摘要
目的观察银杏叶提取物(EGB)对血管性痴呆(VD)小鼠海马生长抑素(SS)和胆囊收缩素(CCK)表达的影响,探讨EGB对VD的改善作用。方法制备VD小鼠模型,利用Y-迷宫测试VD模型小鼠学习记忆能力及高、低EGB对痴呆的改善作用,实时定量聚合酶链反应(PCR)法检测各组动物海马中SS和CCK mRNA的变化,免疫组织化学法研究高、低EGB对VD小鼠海马SS和CCK阳性神经元数量的影响。结果各实验组小鼠均比正常对照组小鼠Y-迷宫学习记忆训练次数明显增多(P<0.05),高、低EGB治疗组迷宫学会次数与模型组比较明显减少,差异有统计学意义(P<0.05),且高EGB治疗组较低EGB治疗组迷宫学习次数明显减少,差异有统计学意义(P<0.05)。VD模型组小鼠海马SS和CCK mRNA转录水平与正常对照组相比显著下调,高、低EGB治疗组与VD模型组相比显著上调,差异均有统计学意义(P<0.05);VD模型组小鼠海马CA1区SS和CCK阳性神经元数量与正常对照组相比显著减少,高、低EGB治疗组与VD模型组相比显著增多,差异均有统计学意义(P<0.05)。结论银杏叶提取物通过增加海马中SS和CCK的表达可一定程度上改善VD小鼠的学习记忆能力。
Objective To observe the effect of ginkgo biloba extract（EGB） on the expression of somatostatin（SS） and cholecystokinin（CCK） in the hippocampus formation of vascular dementia（VD） mice and explore the treatment effect of the EGB on VD. Methods Model of VD mice was prepared, Y-maze test was used to observe the changes in the learning and memorizing abilities and the role of high and low EGB on VD. Real-time polymerase chain reaction（ real-time PCR）was used to detect the changes of expression of SS and CCK mRNA in hippocampus formation of each group mice. Immunohistochemistry were used to observe the effect of high and low EGB on the number of positive neurons of SS and CCK in VD mice. Results The results of Y-maze test suggested that compared with control group, the learning and memory training times of each experimental group was increased （ P 〈 0.05 ）. Compared with model group, the learning times of the low and high EGB treatment groups were decreased（ P 〈 0.05 ）. The learning times of the high EGB treatment group was less than low EGB treatment group （ P 〈 0.05 ）. Compared with the normal control group, the transcriptional levels of SS and CCK mRNA in hippocampus formation of the VD model group was obviously decreased （P 〈 0.05 ）. Compared with the control group, the number of positive neurons of SS and CCK in CA1 zone in the VD model group was obviously decreased（P 〈0.05）. Compared with the VD model group,the number of positive neurons of SS and CCK in CA1 zone in the low and high EGB treatment groups were obviously increased （ P 〈 0.05）. Conclusion EGB can improve the learning and memory ability by increasing the expression of SS and CCK in the hippocampal formation.
出处
《新乡医学院学报》
CAS
2013年第1期24-26,30,共4页
Journal of Xinxiang Medical University
基金
武警后勤学院博士启动金资助项目(编号:WHB201205)
关键词
银杏叶提取物
血管性痴呆
海马
生长抑素
胆囊收缩素
ginkgo biloba extract
vascular dementia
hippocampal formation
somatostatin
cholecystokinin
