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单核细胞趋化蛋白-1基因-2518G/A多态性与2型糖尿病肾病的相关性研究 被引量:6

A correlation research between the monocyte chemoattractant protein-1 gene-2518G/A polymorphism and diabetic nephropathy
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摘要 目的探讨广西汉族人群单核细胞趋化蛋白-1基因-2518G/A(MCP-1-2518G/A)多态性与糖尿病肾病(DN)的关系。方法将130例2型糖尿病患者按DN诊断标准分为DN组与糖尿病非肾病(T2DM)组,另选健康体检者80例作为健康对照组(NC组)。应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析各组基因型及等位基因频率的分布。结果 DN组的收缩压(SBP)、舒张压(DBP)、尿微量清蛋白排泄率(UAER)、肌酐(Cr)、尿素氮(BUN)值均高于NC组及T2DM组(P<0.05)。DN组与T2DM组、NC组之间基因型分布差异有统计学意义(P<0.01),DN组患者GG基因型频率和G等位基因频率均显著高于NC组和T2DM组(P<0.01,P<0.05)。与NC组相比,携带G等位基因者发生DN的相对风险度增加1.406倍(P<0.01,95%CI:1.112~1.777),携带G等位基因的DN患者血清三酰甘油(TG)、总胆固醇(TC)及低密度脂蛋白胆固醇(LDL-C)水平显著高于不携带者(P<0.05)。结论 MCP-1-2518G/A多态性与广西地区汉族人群DN的发病存在相关性,G等位基因可能是DN发病的遗传易感基因。 Objective To explore the relationship between monocyte chemoattractant protein-1 gene -2518G/A polymorphism and diabetic nephropathy (DN) in people of Han group in Guangxi. Methods One hundred and thirty patients with type 2 diabetes mellitus were divided into diabetic nephropathy(DN) group and diabetes without diabetic nephropathy(T2DM) group. Eighty healthy people were selected as normal control(NC) group. The genotypes and allele frequencies distribution were assessed by Polymerase chain reaction-restriction fragment length Polymorphism (PCR-RFLP). Results The values of systolic pressure blood (SBP), diastolic blood pressure (DBP), urine albumin excretion rate (UAER), creatinine (Cr), and blood urea nitrogen (BUN) in DN group were significantly higher than those of NC or T2DM group (P〈0. 05). There was significant difference in the distribution of genotypes and allele frequencies between DN group and NC or T2DM group (P〈0.01). The GG genotypes and G allele frequency distributions in DN group were significantly higher than the NC group and T2DM group (P〈0.01 ,P〈0.05). The G allele carriers had 1. 406 times larger risk for increased incidence of DN compared with healthy controls (P〈0.01,95% CI= 1. 112-- 1. 777). The values of TG,TC and LDL-C were significantly higher among carriers of G allele compared with non-carriers (P〈 0.05). Conclusion The polymorphism of MCP-1-2518G/A is associated with the development of DN in Han nationality in Guangxi province. G allele may be a genetic risk factor incidence of DN.
出处 《重庆医学》 CAS CSCD 北大核心 2013年第4期363-366,共4页 Chongqing medicine
基金 广西卫生厅课题资助项目(Z2005163)
关键词 糖尿病 2型 糖尿病肾病 趋化因子CCL2 多态性 单核苷酸 diabetes mellitus, type 2 diabetic nephropathy chemokine CCL2 polymorphism, single nucleotide
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参考文献17

  • 1Rivero A,Mora C,Muros M. Pathogenic perspectives for the role of inflammation in diabetic nephropathy[J].Clinical Science(London),2009,(06):479-492.
  • 2Ruster C,Wolf G. The role of chemokines and chemokine receptors in diabetic nephropathy[J].Frontiers in Bioscience,2008,(08):944-955.
  • 3Usui HK,Shikata K,Sasaki M. Macrophage scavenge receptor-a-deficient mice are resistant against diabetic nephropathy through amelioration of microinflammation[J].Diabetes,2007,(02):363-372.doi:10.2337/db06-0359.
  • 4Tesch GH. MCP-1/CCL2:a new diagnostic marker and therapeutic target for progressive renal injury in diabetic nephropathy[J].American Journal of Physiology-Renal Physiology,2008,(04):697-701.
  • 5Rovin BH,Lu L,Saxena R. A novel polymorphism in the MCP-1 gene regulatory region that influences MCP-1 expression[J].Biochemical and Biophysical Research Communications,1999,(02):344-348.
  • 6Yang B,Houlberg K,Millward A. Polymorphisms of chemokine and chemokine receptor genes in type 1 diabetes mellitus and its complications[J].Cytokine,2004,(03):114-121.
  • 7Kim HL,Lee DS,Yang SH. The polymorphism of monocyte chemoattractant protein-1 is associated with the renal disease of SLE[J].American Journal of Kidney Diseases,2002,(06):1146-1152.
  • 8Karrer S,Bosserhoff AK,Weiderer P. The -2518 promotor polymorphism in the MCP-1 gene is associated with systemic selerosis[J].Journal of Investigative Dermatology,2005,(01):92-98.
  • 9Lee SH,Ryu JK,Jeong JB. Polymorphisms of the MCP-1 and HSP70-2 genes in korean patients with alcoholic chronic pancreatitis[J].Digestive Diseases and Sciences,2008,(06):1721-1727.doi:10.1007/s10620-007-0049-1.
  • 10Jemaa R,Rojbani H,Kallel A. Association between the-2518G /A polymorphism in the monocyte chemoattractant protein-1(MCP-1) gene and myocardial infarction in Tunisan patients[J].Clin Chmi Acta,2008,(1/2):122-125.

二级参考文献21

  • 1陈施艳,杨期东,杨晓苏,王改青,毛诗贤.MCP-1基因-2518G/A多态性与脑梗死的相关性研究☆[J].中国神经精神疾病杂志,2006,32(1):77-78. 被引量:5
  • 2ROLLINS BJ .Chemokines[J].Blood, 1997,90(3):909-928.
  • 3ROVIN BH., LU L, SAXENA R. A novel polymorphism in the MCP-1 gene regulatory region that influences MCP-1 expression [J].Biochemical and Biophysical Research Communi- cations, 1999,259:344-348,.
  • 4SIMEONI E, HOFFMANN M, WINKELMANN BR, et al. Association between the A-2518G polymorphism in the monocyte chemoattractant protein-1 gene and insulin resistance and Type 2 diabetes mellitus[J].Diabetologia, 2004, 47: 1574-1580.
  • 5KOHZO TAKEBAYASHI, SACHIKO MATSUMOTO,YOSHIMASA ASO,et al.Association between circulating monocyte ehemoattraetant protein-I and urinary albumin excretion in nonobese Type 2 diabetic patients[J]. Diabetes Complications,2006,20:98-104.
  • 6LEE S, KIM B, SIK Y,et al. High glucose induce MCP-1 expression partly via tyrosine-AP-1 pathway in peritoneal mesothlial cells[J]. Kidney Int, 2001, 60(1): 55-64.
  • 7CHIARELLI F, CIPLLONE F, MOHN A, et al. Circulation monocyte chemoattractant protein-1 and early development in nephropathy in type 1 diabetes[J]. Diabetes Care, 2002,25 (10): 1829-1834.
  • 8MANO Y,KASHIWAGI A,NISHIO Y,et al.IDL can stimulate atherogenic gene expression in cultured human vascular endothelial cells [J]. Diabetes Res Clin Pract, 2000, 48 (2): 127-138.
  • 9KEULENAER D,FUKAI U,YIN Q, et al. Convergence of redox-sensitive and milogen activated protein kinase signaling pathways in tumor necrosis factor alpha mediated monocyte chemo-attractant protein-1 induction in vascular smooth muscle cells[J]. Arterioscler Thromb Vasc Biol, 2000, 20(2): 385-391.
  • 10Hong SB,Jin SY,Park HJ,et al.Analysis of the monocyte chemoattractant protein 1-2518 promoter polymorphism in korean patients with alopecia areata[J].J Korean Med Sci,2006,21(1):90.

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