摘要
目的探讨多重耐药铜绿假单胞菌的氨基糖苷类药物的耐药机制。方法分离出50株多重耐药铜绿假单胞菌,PCR法检测4种氨基糖苷类修饰酶(AMEs)基因,即aac(6′)-Ⅰ、aac(6′)-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ的表达情况。并用MLVA基因分型的方法对菌株进行聚类分析。结果 50株铜绿假单胞菌中,4种AMEs基因的总检出率为70%。aac(6′)-Ⅰ、aac(6′)-Ⅱ、ant(3″)-Ⅰ、ant(2″)-Ⅰ的检出率分别为60%、40%、24%、32%。MLVA基因分析聚类结果显示,没有多重耐药铜绿假单胞菌的暴发流行。结论多重耐药铜绿假单胞菌的AMEs基因携带率较高,没有发现其爆发流行。
Objective To explore the resistant mechanism to aminoglycosides among multi-drug resistant Pseudomonas aerugi- nosa（PA） strains. Methods Four kinds of aminoglycosides modification enzymes（AMEs） genes were detected by PCR method in 50 strains of multi-drug resistant PA. Multiple-locus variable-number tandem repeat analysis（MLVA） method was used to analyze the relationship of the strains. Results AMEs genes could be detected in 35 of all 50 PA strains（70%）. The detected rate of the four types of genes was ordered as aac（6r）-I（60%）, aac（6%）-II（40%）, ant（2＂）-I（32%） and ant（3＂） -II（24%）. The result of the MLVA genotype indicated that there was not the fulminate epidemiological feature of multi-drug resistant PA. Conclusion The es- sential mechanisms of aminoglycoside-resistance in PA might be with the producing of AMEs. In addition, the fulminate infection was not found.
出处
《国际检验医学杂志》
CAS
2013年第3期266-268,共3页
International Journal of Laboratory Medicine
基金
中山市科技计划项目(20122A033)
关键词
假单胞菌
铜绿
多重耐药
氨基糖苷类
基因分型
Pseudomonas aeruginosa
multi-drug reisistance
aminoglycosides
genotype
