摘要
骨髓间充质干细胞（BMSCs）是一类位于骨髓基质细胞系中的非造血类成体干细胞。基于BMSCs遗传背景稳定、多向分化潜能、易于获取、容易体外培养与增生，其在组织工程角膜中具有广泛的研究与应用前景。目前大量国内外研究已经证实，BMSCs可以分化为角膜缘干细胞、角膜上皮细胞、角膜内皮样细胞，并且对眼表损害的修复也有重要作用；BMSCs在角膜的应用常借助于某种载体，如异种角膜基质、羊膜等。然而，BMSCs作为组织工程角膜的种子细胞仍存在一些问题需要解决，如多向分化机制尚不明确，BMSCs鉴定尚缺乏统～标准，理想支架材料的选择，细胞大量扩增可能存在致瘤问题等。就BMSCs在组织工程角膜方面的研究进展进行综述。
Bone marrow mesenchymal stem cells （BMSCs） are a group of pluripotential non-hematopoietic somatic stem ceils niched in bone marrow. With the characteristics of stable genetic traits, pluripotential in differentiation,easy to isolate from source tissue, and fast to proliferate when cultured in vitro, BMSCs are currently attracting extensive research interests, and considered to be one of the most promising candidates in corneal tissue engineering. At present, many research groups, domestic and abroad, have reported that BMSCs can not only differentiate into corneal limbal stem cells, corneal epithelial cells, and corneal endothelial ceils, but also play an important role in ocular surface repair. However, the successful application of BMSCs in cornea usually depends on the correct selection of supporting materials or scaffold,such as xenogeneic corneal stroma and amniotic membrane. Other unsolved problems in BMSCs-related corneal tissue engineering include the molecular biologic mechanism underlying the directional differentiation from BMSCs to corneal cells,the standards to identify BMSCs from differentiated corneal cells ,the optimal scaffold materials and the potential tumorigenicity with grafting of transformed or undifferentiated BMSCs. This paper reviewed the progresses and issues of corneal tissue engineering with BMSCs.
出处
《中华实验眼科杂志》
CAS
CSCD
北大核心
2013年第2期196-200,共5页
Chinese Journal Of Experimental Ophthalmology
基金
国务院侨办重点学科项目（51205004）
珠海市科技重点项目（PB20051014）
关键词
骨髓间充质干细胞
组织工程
角膜
支架载体
分化
Bone marrow mesenchymal stem cell
Tissue engineering
Cornea
Scaffold
Differentiation
