摘要
目的:建立改进的同时测定氨酚伪麻那敏片(Ⅱ)中对乙酰氨基酚、盐酸伪麻黄碱、马来酸氯苯那敏含量的HPLC法。方法:采用Inertsil C_(18)柱(150 mm×4.6 mm,5μm),流动相:0.3%十二烷基硫酸钠溶液(含0.2%磷酸溶液)-乙腈(45:55)(用浓氨溶液调节pH至3.9),流速为1.0 ml·min^(-1),检测波长215 nm。结果:对乙酰氨基酚、盐酸伪麻黄碱、马来酸氯苯那敏分别在62～682,6.23～68.56,0.384～4.226μg·ml^(-1)浓度范围内与各自峰面积积分值呈良好的线性关系;平均回收率分别为99.47%(RSD=0.24%),99.33%(RSD=0.34%),99.18%(RSD=0.20%)(n=6)。结论:该方法准确、简便,可用于氨酚伪麻那敏片(Ⅱ)
Objective：To establish an improved HPLC method for the determination of paracetamol,pseudoephedrine hydrochloride and chlorphenamine maleate tablets（Ⅱ）.Method：An Inertsil _（18）（150 mm×4.6 mm,5μm） column was used.The mobile phasewas0.3%sodium dodecyl sulfate solution（including 0.2%phosphoric acid solution）-acetonitrile（45：55）（adjusting pH to 3.9 with concentrated ammonium liquid）.The flow rate was 1.0 ml · min^（-1） and the detection wavelength was at 215 nm.Result：The linear range was 62-682μg· ml^（-1） for paracetamol,6.23-68.56μg ·ml^（-1） for pseudoephedrine hydrochloride and 0.384-4.226μg ·ml^（-1） for chlorphenamine maleate.The average recovery was 99.47%（RSD = 0.24%,n = 6 ）,99.33%（RSD = 0.34%,n = 6） and 99.18%（RSD =0.20%,n=6）,respectively.Conclusion：The method is accurate and convenient,and can be used in the determination of paracetamol,pseudoephedrine hydrochloride and chlorphenamine maleate tablets.
出处
《中国药师》
CAS
2012年第12期1731-1732,共2页
China Pharmacist
