摘要
以姬松茸J1为出发菌株,采用60 Co-紫外线复合诱变,得到1株与原菌株形态差异显著的姬松茸诱变菌株。经出菇试验,诱变菌株在子实体颜色、朵重、盖直径、盖厚、柄直径、柄长度等农艺形状与原菌株均有明显差异,产量大幅提高。生物学特性研究表明,诱变菌株在不同温度、不同pH及不同料水比情况下菌丝的生长速度与姬松茸J1均有很大差异。用扫描电镜观察诱变菌株及出发菌株的菌丝体,诱变菌株菌丝变细,表面有颗粒物,与原菌株有显著差异。经过SSR标记的指纹图谱分析,诱变菌株与原菌株也存在明显差异。研究结果表明,该诱变菌株为一个具有很大开发潜力的新菌株,命名为福姬J77。
In this paper,a strain of agaricus blazei named J1 was used as original strain.Using 60CO-UV compound mutation,a mutant strain was got that had significant differences with J1 in morphology.The result of fruiting experiment showed that the agronomic characters of the mutant strain including color of fruiting body,the average weight per fruitbody,the diameter of pileus,the thickness of pileus,the diameter of stalk,the height of stalk,and so on,was significantly different with J1,and the yield increased significantly.Biological characteristic research indicated∶ the mycelium growth rate of mutant strain and J1 has significant differences in different temperature,pH and ratio of material to water.The mycelium of mutant strain and J1 were observed by means of Scanning Electron Microscopy,and the results showed that the mycelium of mutant strain with particulate on surface was thinner than J1.The analysis of SSR marks fingerprint showed that the mutant strain and J1 had obvious differences.Series of studies showed that the mutant strain which was named ＂FUJI J77＂ is a new strain that has great potential for development.
出处
《福建农业学报》
CAS
2012年第12期1333-1338,共6页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划重点项目(2002N047)
关键词
姬松茸
诱变
60Co-γ射线
紫外线
选育
agaricus blazei
mutation
60Co-γ ray
ultraviolet radiation
breeding
