摘要
通过对从茄假单胞菌中克隆的 p GX12 52进行亚克隆分析 ,发现含 p GX12 52右边4 .5kb K pn I/ Eco RI片段的亚克隆 p GX140 4仍象 p GX12 52一样能使对花生不致病菌株 T2 0 14在花生上致病 .用转座子 Tn5- lac对 p GX140 4进行了饱和插入诱变 ,一共分离得到 6个在 p GX140 4上不同位置的独立插入突变 ,其中离 p GX140 4右末端约 1.4 kb、2 .2 kb的两个 Tn5- lac插入使p GX140 4丧失了扩大 T2 0 14寄主范围的能力 ,证实 hsv基因位于 p GX140
Subcloning analysis of pGX1252 found that 4.5kb KpnI/EcoRI fragment on its right side (pGX1404) still retained the ability of pGX1252 to extend the host range of nonpathogenic potato strain T2014 to include peanut. Plasmid pGX1404 was saturatedly mutated with transposon Tn5lac and six independent insertions of transposon on the insert of pGX1404 were isolated. Two Tn5lac insertions, which were ca.1.4 kb and 2.2 kb away from the right hand end of pGX1404, had made it lose the ability to extend the host range of T2014, indicating that hsv gene(s) was on the right side of pGX1404. The Tn5lac of these two mutants of pGX1404 were markerexchanged to the chromosome of the starting pathogenic strain T2015 generating mutants T2134 and T2135, respectively. Plant tests revealed that the virulence of T2134 and T2135 on peanut was significantly lower than that of T2015, confirming that the transposon disrupted gene(s) of pGX1404 was peanut specific virulence gene.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第3期440-445,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金!(39970 0 11)
广西教育厅人才工程项目
