摘要
目的初步研究144株鲍曼不动杆菌临床分离株药敏结果与脉冲场电泳分子分型、ERIC-PCR指纹图谱分型结果之间的相关性。方法收集2010年10月～2011年12月临床送检标本分离的病原菌是鲍曼不动杆菌的临床菌株144株，运用仪器法（MIC）进行药敏试验，利用脉冲场电泳、ERIC—PCR指纹图谱方法进行分子分型，比对两者结果。结果144株茵株中对14种临床常用抗生素全敏感型34株，全耐药型74株，部分耐药型36株。全敏感型菌株带型数其PFGE结果在lO～13条，全耐药型菌株带型为19条，部分耐药型带型在14～18条；ERIC—PCR指纹图谱分析144株菌株分为12个基因型。结论PFGE分子分型结果可以初步显示耐药情况，相同的分子分型结果药敏结果一致性较高；ERIC-PCR指纹图谱结果与耐药相关性仍需进一步研究。
Objective To research the correlation between the antimicrobial susceptibility and pulsed-field gel electrophoresis molecular classification and ERIC-PCR fingerprinting of 144 strains of acinetobacter baumannii. Methods 144 strains of acinetobacter baumannii were isolated from clinical samples that collected from October 2010 to December 2011, using con- ventional methods for culturing and instrument method （MIC） for drug sensitivity testing,and using pulsed-field gel electro- phoresis method for molecular typing. Then comparied the two results using statistical methods. Results Among the all 144 strains,34 strains sensitive to all 14 clinical commonly used antibiotics,74 strains resistant to all 14 clinical commonly used antibiotics,and 36 strains only resistant to sonic antibiotics. All of strains that sensitive to all antibiotics had 10 to 13 electro- phoresis strips according to the results of PFGE,and that resistance to all antibiotics had 19 electrophoresis strips,and that resistance to some antibiotics had 14 to 18 electrophoresis strips. Using ERIC-PCR fingerprinting, the all 144 strains could be classified as 12 kinds of genotype, Conclusion The drug resistance could be showed by molecular typing preliminarily, and there existed fairly high consistency between tile same results of molecular typing and drug susceptibility testing. But the relation between the results of ERIC-PCR fingerprinting and drug susceptibility testing needs to be studied further.
出处
《现代检验医学杂志》
CAS
2012年第6期67-69,共3页
Journal of Modern Laboratory Medicine
关键词
鲍曼不动杆菌
药敏
脉冲场电泳
分子分型
ERIC—PCR
相关性
acinetobacter baumannii
drug sensitivity test pulsed-field gel electrophoresis
molecular typing
ERIC-PCR
corre-lation
