摘要
目的检测强直性脊柱炎（ankylosingspondylitis，AS）患者的免疫功能，研究其变化及相应临床意义。方法采用流式细胞术分别检测AS组及对照组外周血CD3＋T，CD4＋T，CD8＋T，CDl6＋56＋NK，CDl9＋B细胞占淋巴细胞的百分率，并计算CD4＋／CD8＋比值。免疫散射比浊法测定血清免疫球蛋白IgG，IgA．IgM以及补体C3，C4的含量。结果AS组外周血CD3＋T，CD4＋T，CD8＋T，CDl6＋56＋NK，CDl9＋B细胞百分率及CD4＋／CD8＋比值分别为65．83％±8．50％，38．33％±7．21％，24．07％±8．52％，9．14％±4．90％，15．07％±6．53％和l_85土0．95，血清IgG，IgA，IgM及补体C3，C4的含量分别为14．48±4．00g／L，3．02±1．14g／L，1．53±0．92g／L，1．20±0．22g／L和0．28±0．1lg／L。其中CD3＋T，CD4＋T，CDl9＋B细胞百分率，CD4＋／CD8＋比值及血清IgG，IgA，IgM，C3，C4含量显著高于对照组，且差异具有统计学意义（P〈O．01或P〈O．05），而CDl6＋56＋NK细胞百分率显著低于对照组（P〈0．01）。CD8＋T细胞百分率与正常对照组差异无统计学意义（P〉0．05）。结论AS患者存在细胞免疫、体液免疫以及天然免疫紊乱。检测AS患者免疫功能的变化具有重要临床意义。
Objective To detect the immune functions of patients with ankylosing spondylitis（AS） to study its change and clinical significance. Methods The percentage of CD3＋ T,CD4＋ T,CD8＋ T,CD16＋ 56＋ NK and CD19~ B lymphocytes in pe- ripheral blood from AS and control groups were measured by flow cytometry,and the ratio of CD4＋/CD8＋ was calculated. Meanwhile, the levels of serum immunoglobulin IgG, IgA,IgM and complement C3,C4 were measured by nephelometry. Re- suits The percentage of CD3＋ T,CD4＋ T,CD8＋ T,CD16＋ 56＋ NK,CD19＋ B cells and CD4＋/CD8＋ ratio in peripheral blood of AS group were 65.83%±8.50%,38.33%±7.21%,24.07%±8.52%,9.14%±4. 90%,15.07%±6.53% and 1.85±0.95 respectively,and the levels of serum IgG,IgA,IgM,C3 and C4 were 14.48±4.00 g/L,3.02±1.14 g/L,1.53±0.92 g/L,1.20±0.22 g/L,0.28±0.11 g/L respectively. Compared with control groups,the percentage of CD3＋ T,CD4＋ T and CD19 ＋ B cells,the ratio of CD4＋/CD8＋ ,and the levels of serum IgG, IgA, IgM, C3 and C4 in AS were significantly higher （P〈0.01 or P〈0.05） ,while the percentage of CD16＋ 56＋ NK cells were dramatically lower （P〈0.01）. There were no sta- tistical difference of the percentage of CD8＋ T cells between AS patients and normal controls （P〉0.05）. Conclusion Cellu- lar and humoral immunity as well as innate immunity were significantly disorder in patients with AS. Detection of the im- mune functions change has important clinical significance for patients with AS.
出处
《现代检验医学杂志》
CAS
2012年第6期132-134,共3页
Journal of Modern Laboratory Medicine
关键词
强直性脊柱炎
淋巴细胞亚群
免疫球蛋白
补体
ankylosing spondylitis （AS） lymphocyte subsets
immunoglobulin complement
