摘要
目的探讨IL-10和IL-20在腰椎间盘突出症模型大鼠的表达。方法 50只SD雄性大鼠随机均分成5组。实验组大鼠在腰椎间盘突出症模型建立后分别于1、2、3、4周(1、2、3、4组)处死,ELISA法测定外周血、髓核及周围组织中IL-10、IL-20含量,并取髓核组织行免疫组化染色和显微镜观察。结果与对照(C组)大鼠比较。结果 1组和2组血清IL-10表达为(34.69±10.76)和(32.13±11.78),明显高于C组的(25.27±7.59)(P<0.05);血清IL-20表达为(8.98±1.25)和(5.42±2.05),也明显高于C组的(3.06±0.95)(P<0.05)。1组和2组髓核组织中IL-10表达为(31.35±8.69)和(25.97±6.31),明显高于C组的(20.60±5.89)(P<0.05);髓核组织中IL-20表达为(30.73±3.82)和(18.72±3.48),也明显高于C组的(15.02±1.89)(P<0.05)。1、2组髓核组织中均发现有IL-10和IL-20阳性表达的细胞。结论在腰椎间盘突出症的急性炎症反应期中,大鼠血清、髓核及周围组织中均有IL-10和IL-20的表达;IL-10和IL-20可能参与了髓核炎性反应。
Objective To investigate the expressions of interleukin（IL）-10 and IL-20 in the rat models with lumbar disc herniation（LDH）. Methods Fifty male SD rats were equally randomized into 5 groups.After LDH models were established,the rats were killed at 1（group A）,2（group B）,3（group C） or 4 weeks（group D）, respectively.The expressions of IL-10 and IL-20 in the nucleus pulposus and its adjacent tissues and serum were detected by immunohistochemical staining and ELISA. Results The expressions of IL-10 in serum were higher in groups of A and B than those in group C [（34.69±10.76） and（32.13±11.78） vs.（25.27±7.59）]（P〈0.05） and so did those of IL-20 [（8.98±1.25） and（5.42±2.05） vs.（3.06±0.95）]（P〈0.05）.The expressions of IL-10 in the nucleus pulposus and its adjacent tissues were higher in groups of A and B than those in group C [（31.35±8.69） and（25.97±6.31） vs.（20.60±5.89）]（P〈0.05） and so did those of IL-20 [（30.73±3.82） and（18.72±3.48） vs.（15.02±1.89）]（P〈0.05）. The cells with IL-10 and IL-20 expressions in the nucleus pulposus and its adjacent tissues were seen in groups of A and B. ConclusionThe expressions of IL-10 and IL-20 in serum and nucleus pulposus and its adjacent tissues are higher in LDH rat models in acute phase,which may participate in the inflammatory reaction of pulposus.
出处
《江苏医药》
CAS
北大核心
2013年第2期145-147,F0002,共4页
Jiangsu Medical Journal
