摘要
目的:提取和纯化人参皂苷Rg1,并测定其含量。方法:采用超声法提取人参总皂苷,使用硅胶柱层析法分离、纯化人参皂苷Rg1,采用高效液相色谱法测定人参皂苷Rg1含量。结果:采用硅胶柱层析分离50g人参,可得到人参皂苷Rg19.91g,其纯度为89.63%。结论:此提取方法简单、准确,成本较低,所获产品纯度较高,可作为获得人参皂苷Rg1的有效方法。
OBJECTIVE： To extract and purification ginsenoside Rgl and to determine the content of it. METHODS： Ultrasonic extraction was used to extract total saponin of Panax ginseng. Ginsenoside Rg1 was separated and purified with silica gel column chromatography. HPLC method was used to determine the content of ginsenoside Rg1. RESULTS： 9.91 g ginsenoside Rg1 was ob- tained with the purity of 89.63% in 50 g P. ginseng. CONCLUSION： The method is simple, accurate and low cost, and the purity is satisfied. So, this technology is suitable to prepare ginsenoside Rg1.
出处
《中国药房》
CAS
CSCD
2013年第7期605-607,共3页
China Pharmacy
基金
重庆市科技计划项目资助(No.CSTC
2009BA5086)
