摘要
目的初步探讨腹泻型肠易激综合征(IBS)患者是否存在辅助性T细胞1(TH1)、辅助性T细胞2(TH2)免疫调节功能紊乱及其在IBS发病中的作用。方法应用酶联免疫吸附试验(ELISA法)检测31例腹泻型IBS患者(IBS组)及28例同龄、同性别、同期健康者(对照组)血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-4、IL-8、IL-10、IL-12、干扰素(IFN)-γ水平。结果腹泻型IBS患者血清中可测得低浓度TNF-α,对照组血清中未检测到TNF-α;腹泻型IBS患者血清IL-4、IL-10水平与对照组比较,差异无统计学意义(P>0.05);腹泻型IBS患者血清IL-12、IFN-γ、IL-8水平明显高于对照组,两组比较,差异有统计学意义(P<0.01);腹泻型IBS患者IFN-γ/IL-4比值明显高于对照组,差异有统计学意义(P<0.01)。结论腹泻型IBS患者体内TH1反应增强,感染影响腹泻型IBS患者发生TH1/TH2漂移,在IBS发病过程中起重要作用。
Objective To primarily investigate the possible disturbance of TH1/TH2 immunoregulation in the patients with diarrhea-predominant irritable bowel syndrome（D-IBS） and its role in the pathogenesis of IBS.Methods The expressions of serum tumor necrosis factor（TNF）-α,interferon（IFN）-γ,interleukin（IL）-12,IL-4,IL-8 and IL-10 in 31 patients with D-IBS（IBS group）and contemporaneous 28 healthy people with the same age and sex（the control group）were determined with enzyme-linked immunosorbent assays（ELISA）.Results Low level of serum TNF-α was detected in the D-IBS group,while serum TNF-α was undectable in the control group;the levels of serum IL-4 and IL-10 in the D-IBS group had no statistical differences compared with those in the control group（P0.05）;the levels of IL-12,IFN-γ and IL-8 in the D-IBS group were increased more significantly than those in the control group with statistical differenc（P0.01）;the ratio of IFN-γ/ IL-4 in the D-IBS group was significantly higher than that in the control group with statistical difference（P0.01）.Conclusion The TH1 response is enhanced and infection affects the shift of TH1/TH2 in the patients with D-IBS,which plays an important role in the pathogenesis of IBS.
出处
《现代医药卫生》
2013年第2期180-181,共2页
Journal of Modern Medicine & Health
