摘要
目的探讨香椿子正丁醇提取物对脑缺血再灌注致大鼠多器官功能障碍综合征的保护作用。方法大鼠随机分为正常对照组,假手术组,模型组,溶媒对照组,香椿子正丁醇提取物低、高剂量(20、30 mg/kg)组,阿司匹林(10 mg/kg)阳性对照组,各给药组每天给药1次,连续给药7 d。第8天以大脑中动脉线栓法制备大鼠局灶性脑缺血再灌注模型,造模后,行神经行为学评分判断神经功能损伤程度,伊文思蓝法观察血脑屏障通透性,分光光度法测定胃肠组织中丙二醛(MDA)、一氧化氮(NO)的量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性。结果与模型组相比,香椿子正丁醇提取物可以改善脑缺血再灌注大鼠神经功能损伤(P<0.05)、降低血脑屏障通透性(P<0.05);减少胃和小肠组织MDA和NO的生成(P<0.05),抑制SOD、GSH-Px活性升高(P<0.05)。结论香椿子正丁醇提取物通过抗氧化应激效应对脑缺血再灌注致多器官功能障碍综合征发挥保护作用。
Objective To investigate the protective effects of n-butanol extract from the seeds of Toon sinensis （NESTS） on multiple organ dysfunction syndrome （MODS） caused by brain ischemia-reperfusion in rats. Methods Male SD rats were divided into seven groups, such as control, Sham, model, vehicle control, Aspirin （10 mg/kg, positive control）, low- and high-dose （20 and 30 mg/kg） NESTS groups. The rats in the treated groups were ig administered once daily for 7 d. On the day 8 the focal cerebral ischemia-reperfusion rat model was established using middle cerebral artery suture method. After the model establishment, the neurological deficit score was evaluated; The permeability of the blood brain barrier （BBB） was evaluated by Evans Blue （EB） staining; The levels of malondialdehyde （MDA） and nitrogen oxide （NO）, and the activities of superoxide dismetase （SOD） and glutathione peroxidase （GSH-Px） in gastrointestinal tissue were detected by spectrophotometer. Results NESTS improved the neurological deficit （P 〈 0.05）, reduced the permeability of BBB （P 〈 0.05）, decreased the production of MDA and NO in gastrointestinal tissue, and inhibited the increase of SOD and GSH-Px activities （P 〈 0.05）. Conclusion NESTS could alleviate the MODS caused by brain ischemia-reperfusion in rats, which is partly mediated by its anti-oxidative stress effect.
出处
《中草药》
CAS
CSCD
北大核心
2013年第3期323-326,共4页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金青年基金项目(81100873)
湖北省卫生厅青年科技人才项目(QJX2010-27)
三峡大学博士科研启动基金项目(KJ2008B053)
关键词
香椿子
脑缺血再灌注
多器官功能障碍综合征
抗氧化应激
血脑屏障
seeds of Toon sinensis
brain ischemia-reperfusion
multiple organ dysfunction syndrome
anti-oxidative stress
blood brain barrier
