摘要
目的建立白芨愈伤组织诱导、增殖和分化再生体系。方法以MS为基本培养基,应用正交试验方法设计植物生长调节剂组合和质量浓度配比,研究不同外植体及植物生长调节剂对白芨愈伤组织形成、增殖和分化的影响。结果白芨假鳞茎为诱导愈伤组织形成的最佳外植体;1.0 mg/L 6-苄氨基嘌呤(6-BA)与2.0 mg/L 2,4-二氯苯氧乙酸(2,4-D)组合不仅可诱导白芨愈伤组织形成,还有利于愈伤组织分化成芽。1.0 mg/L 6-BA与3.0 mg/L 2,4-D的组合促进愈伤组织增殖,增殖系数为7.8;在分化培养基中附加0.5 mg/L噻二唑苯基脲(TDZ),可提高愈伤组织分化率。结论白芨愈伤组织诱导的最佳培养基为MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L;最佳增殖培养基为MS+6-BA 1.0 mg/L+2,4-D 3.0 mg/L;分化最佳培养基为MS+6-BA1.0 mg/L+2,4-D 2.0 mg/L+TDZ 0.5 mg/L。
Objective To establish the regeneration system ofBletilla striata callus for induction, proliferation, and differentiation. Methods MS was selected as the basic medium. To study the effective factors on induction, proliferation, and differentiation of callus ofB. striata with different explants and to optimize the plant growth regulators, kinds, and concentration ratio of plant growth regulators by orthogonal test design. Results The optimal explants were false bulbs ofB. striata. MS medium added with 1.0 mg/L 6-benzylaminopurine （6-BA） and 2.0 mg/L 2, 4-dichlorophenoxy acetic acid （2, 4-D）, not only could induce callus formation, but also benefit to the differentiation of callus. MS medium added with 1.0 mg/L 6-BA and 3.0 mg/L 2, 4-D promoted the proliferation of callus, and the proliferation coefficient was 7.8. Adding with 0.5 mg/L thidiazuron （TDZ） to the differentiation medium improved the differentiation rate of callus. Conclusion MS ＋ 6-BA 1.0 mg/L ＋ 2, 4-D 2.0 mg/L is the optimal medium for the induction of callus; MS ＋ 6-BA 1.0 mg/L ＋ 2, 4-D 3.0 mg/L is the optimal medium for the proliferation of callus; MS ＋ 6-BA 1.0 mg/L ＋ 2, 4-D 2.0 mg/L ＋ TDZ 0.5 mg/L is the optimal medium for the differentiation of callus.
出处
《中草药》
CAS
CSCD
北大核心
2013年第3期349-353,共5页
Chinese Traditional and Herbal Drugs
基金
广西自然科学基金项目"药用植物白芨多倍体育种技术研究"(2011GXNSFA018105)
广西科技攻关项目"广西重要兰科资源植物保护与繁育技术研究"(桂科攻10100012-5)
桂林科技攻关项目"药用植物白芨种源基地建立与产业化研究"(20120105-11)
关键词
白芨
愈伤组织
诱导
增殖
分化
正交设计
Bletilla striata （Thunb.） Reichb. f.
callus
induction
proliferation
differentiation
orthogonal design
