摘要
目的观察血必净注射液对急性百草枯中毒大鼠IL-1、IL-6、TNF-α的影响,探讨血必净注射液对百草枯中毒炎症反应是否有抑制作用。方法将30只雄性Wistar大鼠随机分为Ⅰ组(空白对照组)、Ⅱ组(百草枯染毒组)、Ⅲ组(血必净治疗组),每组10只。对照组大鼠给予生理盐水10 mL/kg灌胃,其余两组分别给予百草枯20 mg/kg灌胃染毒,染毒后30 min开始,Ⅰ、Ⅱ组大鼠给予生理盐水10 mL/kg,1次/d腹腔注射,直至处死;Ⅲ组大鼠给予血必净10 mL/kg,1次/d腹腔注射,直至处死。每日观察大鼠的生命状态,是否有死亡。分别于3、7、14 d采集大鼠内眦静脉血1.5 mL,高速离心,取上层血清,用酶联免疫吸附法检测细胞白介素1(IL-1),细胞白介素6(IL-6),肿瘤坏死因子-α(TNF-α)的表达水平,分析三组实验数据,探讨三组数值之间的相关性。结果①染毒组、血必净治疗组IL-1、IL-6、TNF-α表达水平较空白组明显升高,差异有统计学意义(P<0.001);②血必净治疗组IL-1、IL-6、TNF-α表达水平较染毒组明显降低,差异有统计学意义(P<0.001)。结论在急性百草枯中毒时,引起体内炎性因子增加,导致多脏器功能受损,而血必净注射液有效降低了内毒素及过量炎性介质的损伤,抑制IL-1、IL-6、TNF-α的升高,疗效较明显,为临床治疗提供了有力的证据。
Objective To observe the influence of xuebijing injection on IL-1, IL-6 ,TNF-α in acute paraquat rats, and discuss whether xuebijing injection can inhibit the inflammatory response cased by paraquat poisoning. Meth-ods 30 male Wister rats were randomly divided into control group, poisoning control group, xuebijing treatment group, 10 rats each group. Control group was given saline （10 mL/kg i. p. ） ; poisoning control group and xuebijing treatment group were given paraquat （20 mg/kg i. p. ）. After 30 minutes, control group and poisoning control group were given saline （10 mL/kg）once a day by intraperitoneal injection, xuebijing treatment group was given xuebijing （10 mL/kg） once a day by intraperitoneal injection. To collect 1.5 mL blood from inner and canthal vein on the 3rd day, 7th day, 14th day. To separate serum and detect the expression level of IL-1 ,IL-6 ,TNF-α and analyze the experimental datas and discuss the correlation of the three groups. Results The expression levels of IL-1 ,IL45 ,TNF-α of poisoning control group and xuebijing treatment group were obviously higher than those of control group （P 〈 0. 001 ）. The ex-pression levels of IL-1 ,IL-6 ,TNF-α of xuebijing treatment group were lower than those of poisoning control group（ P 〈 0. 001 ）. Conclusion Inflammatory factors increase in paraquat poisoning, which can cause multiple organ failures, but xuebijing can reduce the injury caused by inflammatory mediators and inhibit the increasing of IL-1, IL-6 and TNF-α. Which provide a basis for future clinical therapy.
出处
《实用药物与临床》
CAS
2013年第1期17-19,共3页
Practical Pharmacy and Clinical Remedies
