摘要
目的采用反相高效液相色谱法（RP-HPLC）分析不同产地西青果中有效成分没食子酸的含量。方法以ZORBAX SB-C18（250mm×4．6min，5μm）色谱柱，以乙腈-0．1％磷酸溶液（29：71）作为流动相，流速为1．0ml／min，柱温：30℃；检测波长：273nm。结果没食子酸在20min内得到较好分离，在0．5611-5．611μg范围内有良好的线性关系，平均加样回收率为99．9％，RSD为2．35％。结论本方法简便，准确，重复性好，为西青果的进一步开发和利用提供依据。
Objective To establish an HPLC method for determining gallic acid in Chebulae fructus immaturus of different area. Methods The ZORBAX SB-C18 （250 mm× 4.6 mm, 5 μm） column was used, the mobile phase consisted of acetonitrile： 0.1% H3PO4（29 ： 71）, the flow rate was 1.0 ml/min, the column temperature was 30℃ the detecting wavelength was at 273 nm. Results Gallic acid was successfully separated within 20 rain, the linear response range was 0.5611-5.611μg. The average recovery was 99.9%, and RSD was 2.35%. Conclusion The method is simple, accurate and repeatable; it can provide evidence for further development and utilization of this crude drug.
出处
《国际中医中药杂志》
2013年第2期140-142,共3页
International Journal of Traditional Chinese Medicine
