摘要
为了研究小麦籽粒中结合态脱氧雪腐镰刀菌烯醇(DON)毒素产生规律,以小麦籽粒为原料,采用人工接种禾谷镰刀菌Fg18.7产毒,三氟甲烷磺酸水解后ELISA检测结合态DON。结果表明:15℃、20℃时游离态DON含量均随着培养时间的延长逐渐增加,25℃时游离态DON含量先增加后减少;不同温度下结合态DON含量均随着培养时间的延长逐渐增加,15℃时40天后达到最高含量为129.20ng·g-1。认为禾谷镰刀菌在小麦籽粒中先产生游离态DON,之后游离态DON会与籽粒中某些成分结合产生不可溶的结合态DON。
In this paper,bound Deoxynivalenol（DON） production by Fusarium graminearum in wheat grain was evaluated.After being hydrolyzed by trifluoromethanesulfonic acid（TFMSA）,bound DON production in wheat grains,which were inoculated artificially with F.graminearum 18.7,was determined by ELISA.The results showed that the content of free DON increased gradually with time at 15℃and 20℃,but it increased maximum and then decreased at 25℃.However,the content of bound DON increased gradually with time at all culture temperatures.And the highest bound DON content was 129.20 ng·g-1 at 15℃ after 40 days.So it can be considered that F.graminearum 18.7 initially produced free DON which then was conjugated with compositions in wheat grain to form insoluble bound DON.
出处
《核农学报》
CAS
CSCD
北大核心
2013年第1期56-60,共5页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(30871750)
