摘要
建立了高效液相色谱-蒸发光散射测定瓜蒌中L-瓜氨酸含量的实验方法。色谱分离使用Agilent ZORBAXSB-Aq柱(250mm×4.6mmi.d.,5μm),流动相为水,流速为1.0mL/min。柱后流出物采用蒸发光散射检测器检测,飘移管温度为115℃,空气做载气,流速为3.2L/min。L-瓜氨酸的峰面积的对数值与质量浓度的对数值呈良好的线性关系,线性范围为0.05～0.75mg/mL,检出限为12.9μg/mL,加标回收率为98.36%,相对标准偏差为1.77%。将该法用于实际样品的测定,结果表明瓜蒌中L-瓜氨酸的含量为0.1mg/g左右。该方法简单、快速、准确、重现性好,适用于瓜蒌药材中L-瓜氨酸的定量分析。
A high performance liquid chromatography- evaporative light scattering detection method for separation and determination of L-citrulline in Trichosanthis Fructus was established. The analytical column was Agilent ZORBAX SB-Aq（ 250mm × 4.6mm i.d.,5 μm） .The mobile phase was water,and the flow rate was 1.0mL /min.The sample was determined by an evaporative light scattering detector.The temperature of drift tube was kept at 115℃ and the flow rate of atmosphere was 3.2L /min.The result showed that under the optimized conditions,the logarithm correlation between peak area and the concentration of L-citrulline was in a good linearity. The calibration curve had good linearity in the range of 0.05 ～ 0.75mg /mL,the detection limit of L-citrulline was 12.9 μg /mL,and the average recovery was 98.36% （ n = 5,RSD = 1.77% ） . The method was applied in the detection of L-citrulline in Trichosanthis Fructus,and the result revealed that the content was about 0.1mg /g. The method is a convenient, rapid and effective method for qualitatively analysis of L-citrulline in Trichosanthis Fructus.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第3期314-316,共3页
Science and Technology of Food Industry
基金
"华中区域中药材规范化种植及大宗中药材综合开发利用技术研究山东省科技发展计划项目"课题(2011BAI06B00)
"山东道地药材资源质量控制科技平台建设"课题(2008GG2NS02022)
关键词
L-瓜氨酸
瓜蒌
高效液相色谱
蒸发光散射检测
L-citrulline
Trichosanthis Fructus
high performance liquid chromatography
evaporative light scattering detection
