摘要
目的:研究桃红四物汤含药血清对过氧化氢损伤的人脐静脉内皮细胞(HUVECs)的保护作用。方法:SD大鼠灌胃给予桃红四物汤提取液(生药1.75 g.mL-1),每天2次,按10 mL.kg-1连续给药3 d后,制备桃红四物汤含药血清。MTT法考察桃红四物汤含药血清(终浓度为5%,10%,15%)预处理对H2O2诱导细胞活力减少的作用;显微镜观察细胞形态学变化;以丙二醛(MDA)和乳酸脱氢酶(LDH)含量、超氧化物歧化酶(SOD)活性以及AO/EB染色考察桃红四物汤含药血清对HUVECs凋亡及抗氧化能力的影响;Western blot法检测细胞Caspase-3的表达。结果:与空白血清对照组比较,经400μmol.L-1H2O2刺激后细胞活性显著降低,含药血清组可显著提高SOD活力、降低MDA和LDH含量,并抑制Caspase-3表达。AO/EB染色及相差倒置显微镜也观察到桃红四物汤含药血清能降低H2O2诱导的细胞凋亡。结论:桃红四物汤含药血清对H2O2诱导的HUVECs损伤有显著地保护作用。
Objective ： To study the protective effect of medicated serum prepared with Taohong Siwu Tang on hydrogen perox- ide-injured human umbilical vein endothelial cells （HUVECs）. Method： Spargue Dawlcy rats were orally administered with 10 mL kg-lextracts from Taohong Siwu Tang （ 1.75 g crude drug） , twice a day for three days, in order to prepare medicated serum of Tao- hong Siwu Tang. The effect of medicated serum pre-treated with Taohong Siwu Tang （with concentrations of 5% , 10% , 15% ） on re- duction of H2Oz-induced cell activity was detected MTF. Cell morphological changes were observed under microscope. The effect of medicated serum prepared with Taohong Siwu Tang on the apoptosis and antioxidant capacity of HUVECs was detected with the conteqt of nmlondialdehyde （MDA） and dehydrogenase （LDH）, the activity of superoxide dismutase （SOD） and AO/EB staining. The ex- pression of Caspase-3 was determined by western blot. Result： Compared with the blank serum control group, cell were significantly less active after being damaged by H202 （400 μmol L-1）. Medicated serum could significantly improve the SOD activity, reduce the levels of LDH and MDA, and inhibite the expression of Caspase-3. AO/EB staining and inverted microscope also showed that medica- ted serum prepared with Taohong Siwu Tang could reduce cell apoptosis induced by H2O2. Conclusion： Medicated serum prepared with Taohong Siwu Tang has significant protective effect on HUVECs injured by H2O2.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第3期402-406,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81073091)
安徽省高校自然科学基金项目(KJ2011A189)
安徽高校优秀人才基金项目(2010SQRL10)
关键词
桃红四物汤
含药血清
人脐静脉内皮细胞
过氧化氢
Taohong Siwu Tang
medicated serum
human umbilical vein endothelial cell
hydrogen peroxide
