摘要
目的探索建立一种稳定且培养成功率高,操作简便的产前绒毛染色体制备的方法,提高绒毛产前诊断的成功率。方法 181例进行产前诊断的绒毛组织分两组,采用胶原酶分别消化10min和15min,制备绒毛细胞悬液进行细胞培养、染色体核型分析。结果 181例绒毛组织培养成功179例,失败2例,培养成功率为98.90%,平均培养时间为10-12天;消化15min比消化10min细胞更容易贴壁,原代培养成功率更高。179例培养成功的绒毛组织检出异常核型14例,其中镶嵌型5例,检出染色体多态性15例。结论采用胶原酶消化绒毛组织,可以提高细胞培养成功率,提高孕早期产前诊断的成功率,适合各级有条件的医疗保健机构推广应用。
Objective：To establish a method of prenatal chorionic villus samples chromosome preparation which is stable,higher success rate and easier to prepare,enhance the success rate of villus prenatal diagnosis.Methods： 181 cases of chorionic villus samples were divided into two groups,digested by collagense for 10 minutes and 15 minutes respectively,and the resulting individual cell suspension were used to establish primary cultures for cytogenetic analysis.Results： 179 of 181 cases were cultured successful and 2 of 181 cases failed.Culture success rate was 98.9%,the average of culture time was 10-12 days.Collagenase digestion for 15 minutes was superior to 10 minutes in adherent cultures,the success rate of primary culture was higher.14 cases of chromosomes aberration and 15 cases of chromosome polymorphism were detected in 179 cases of successful culture,and 5 cases chromosome mosaicism of 14 cases chromosomes aberration.Conclusion： The application of collagenase digestion for the chorionic villus tissue could improve success rate of the chorionic villus cell culture and prenatal diagnosis in the first trimester,it was worth to be extend.
出处
《中国优生与遗传杂志》
2013年第2期36-38,共3页
Chinese Journal of Birth Health & Heredity
基金
广西科学研究与技术开发计划项目
编号:桂科攻0993003A-9
关键词
胶原酶消化
绒毛组织
培养
染色体
产前诊断
Collagenase digestion
Chorionic villus
Culture
Chromosomes
Prenatal diagnosis
