摘要
目的观察microRNA-196a（miR-196a）抑制序列转染胰腺癌细胞株PANC1后对其HOXB8基因表达的影响。方法将PANC1细胞分为对照组、miR-196a抑制序列组和siRNA对照组。采用脂质体法将miR-196a抑制序列及对照siRNA分别转染PANC1细胞。应用RT—PCR和蛋白质印迹法检测转染细胞miR-196a及其下游靶基因HOXB8mRNA和蛋白的表达。结果转染miR-196a抑制序列后，PANC1细胞miR-196a表达量较siRNA对照组显著减少（0．050±0．054比0．839±0．025。t=3．12，P〈0．05）；HOXB8mRNA表达量较siRNA对照组增高1．57倍（2．20±0．07比1．29±0．10，t=3．86，P〈0．05）；HOXB8蛋白表达量也显著增强（0．90±0．03比0．40±0．10，t=3．11，P〈0．05）。结论miR-196a可以下调HOXB8基因的表达。
Objective To investigate the effects of microRNA-196a（miR-196a） inhibitory sequences transfection on HOXB8 expression in PANC1 cells. Methods PANC1 cells were divided into control group, miR-196a inhibitory sequences group and siRNA control group. Liposomal transfection method was applied to transfect miR 196a inhibitory sequences and siRNA control into PANC1 cells. RT-PCR and Western blot were used to detect the expressions of miR-196a and HOXB8 mRNA and protein. Results After miR-196a inhibitory sequences transfeetion, when compared with that of siRNA control group, the expression of miR-196a was significantly decreased （0.05 ± 0. 054 vs. 0. 839 ± 0. 025, t = 3.12, P 〈0.05 ） ; and the expression of HOXB8 mRNA was significantly increased by 1.57 folds （2.20± 0.07 vs. 1.29± 0.10, t = 3.86, P 〈 0.05 ）, the expression of HOXB8 protein was also obviously increased （0.90 ± 0. 03 vs. 0. 40 ± 0.10, t = 3.11, P 〈 0.05 ）. Conclusions MicroRNA-196a down-regulates the expression of HOXB8.
出处
《中华胰腺病杂志》
CAS
2013年第1期13-15,共3页
Chinese Journal of Pancreatology
基金
国家自然基金面上项目（30971344）
上海市科委基础研究重点项目（11JC1416402）
