摘要
目的:了解南方红豆杉内生细菌类群及其系统发育关系;方法:对分离菌株进行形态学、生理生化和分子鉴定,并做系统发育分析。结果:从南方红豆杉茎、皮和叶中共分离到内生细菌52株。对12株内生细菌16S rDNA进行PCR扩增,扩增产物大小约为1 400 bp,对11株内生细菌16S rDNA测序结果,用BLAST软件进行相似性比对,发现TB02株为Enterobacter属,相似性99%;9株为Bacillus属,相似性99%～100%;TB13株为Lysinibacillus,相似性97%。通过构建系统发育树发现这11株内生细菌明显聚为两大支。结论:11株内生细菌分别鉴定为肠杆菌、芽孢杆菌和Lysinibacillus,芽孢杆菌为南方红豆杉内生细菌优势菌属。芽孢杆菌和Lysinibacillus亲缘关系较近,二者和肠杆菌之间亲缘关系较远。
Objective：Tbe endophytic bacteria composition of Taxus chinensis var mairei was investigated. Method：Identification to these i- solates was carried out with morphology, biochemistry and molecular biology methods, respectively. The phylogenetic analysis was carried out, too. Result：In total 52 of endophytic bacteria were isolated from Taxus chinensis var mairei. Result：Of 16S rRNA gene amplification by PCR method shown that about 1 400 bp length of DNA fragments were amplified. A comparative analysis of the 16S rRNA gene se- quence demonstrated that strain TB02 was closely related to Enterobacter sp. $60（99 % similarity）, strain TB13 was closely related to Ly- sinibacillus sp. FA（97 % similarity）, and the other nine strains were to closely related Bacillus genus（99% or 100% similarity）. The e- leven isolates clustered two main branches in evolutionary tree constructed. Condusion：BaciUus was the dominant genus. The evolutionary distance between Bacillus and Lysinibacillus was closer than that between them and Enterobacter.
出处
《生物技术》
CAS
CSCD
北大核心
2012年第5期61-65,共5页
Biotechnology
基金
贵州省科学技术基金项目("贵州石漠化环境中宏基因组文库的构建"
No.20082103)资助
关键词
南方红豆杉
内生细菌
分离鉴定
系统发育分析
Taxus chinensis var mairei
endophytic bacteria
isolation and identification
phylogenetic analysis
