摘要
目的建立UV法检测润肠通便类保健食品中总蒽醌的含量。方法采用UV-2600紫外分光光度计,检测波长为504 nm。以1,8-二羟基蒽醌为对照品,测定吸光度,绘制标准曲线计算含量。结果 1,8-二羟基蒽醌在0.004 2～0.021 0 mg.mL-1线性关系良好,回归方程Y=38.78X-0.008,r=0.999 7。平均回收率为101.5%,RSD为1.0%。结论本方法可用于快速测定润肠通便类保健食品中总蒽醌的含量,操作简便、无需多种对照品、准确可靠。
Objective To establish a UV method to determine total anthraquinone in health food. Methods UV-2600 spectrophotometer was used. The detection wavelength was 504 nm.The assay of content was calculated by external stan- dard method. Results The linear range of 1,8-dihydroxy anthraquinone calibration curve of sorbic acid was 0.004 2 --0.021 0 mg · mL ^- 1 （r = 0.999 7）. The recovery was 101.5% and RSD 1.0%. Conclusion It can be used to determine the content of total anthraquinone in health food. The method is simple, sensitive, accurate and reliable.
出处
《中南药学》
CAS
2012年第12期925-927,共3页
Central South Pharmacy
