摘要
目的探讨失血性休克大鼠早期外周血、脾脏及骨髓中髓系抑制性细胞(MDSCs)的初步变化规律。方法将14只SD大鼠随机分为两组:对照组和失血性休克组,每组7只。失血性休克组大鼠建立重度失血性休克模型,模拟急救流程,即分为失血期、早期复苏期、急救期和观察期,采用双抗体标记流式细胞分析技术测定对照组和失血性休克组大鼠外周血、脾脏和骨髓中的MDSCs含量,并加以比较和分析。结果与对照组相比较,SD大鼠在失血性休克-复苏早期,外周血中的MDSCs含量明显升高,骨髓中的MDSCs含量明显减少,差异均具有统计学意义(P均<0.05)。而脾脏中的MDSCs含量,两组差异无统计学意义(P<0.05)。结论在失血性休克/复苏/急救后的早期阶段,MDSCs即从骨髓向外周血(效应部位)迁徙,从而发挥其免疫效应。
Objective To evaluate the early changes of myeloid-derived suppressor cells （MDSCs） of peripheral blood, spleen and marrow in rats of hemorrhagic shock. Methods Fourteen rats were randomly divided into control and hemorrhagic shock （HS） groups （seven in each group）. A model of severe hemorrhagic shock was established, simulating the whole first-aid process of traumatic shock. MDSCs were analyzed by flow cytometric staining of CDllb/c＋Gra＋ in peripheral blood mononuclear cells （PBMC）, spleen cell suspensions, and bone marrow nucleated cells （BMNC）. Results In the early stage after hemorrhagic shock, fluid resuscitation and emergency treatment, the MDSCs in PBMC of HS group markedly increased, and MDSCs in BMNC of HS group decreased accordingly, significantly different to the control group （all P 〈 0.05）. However, there was no statistically significant difference in MDSCs of spleen between the two groups （P〉 0.05）. Conclusions In the model of rats with severe and controlled hemorrhagic shock, it may cause dramatic early migration and redistribution of MDSCs from bone marrow to peripheral circulation （effector site）. But the evaluation on what and how they affect the immune system and mortality needs further investigation.
出处
《中华危重症医学杂志（电子版）》
CAS
2012年第6期5-9,共5页
Chinese Journal of Critical Care Medicine:Electronic Edition
基金
温州市科技计划项目(Y20100355)
浙江省教育厅科研项目(编号:Y201019154)
浙江省自然科学基金项目(Y2100430)
国家自然科学基金项目(编号:81272075)
关键词
失血性休克
髓系抑制性细胞
流式细胞术
Shock, hemorrhagic
Myeloid-derived suppressor cells
Flow cytometry
