摘要
目的:优选大孔树脂分离、纯化枳壳总黄酮的工艺条件。方法:通过静态吸附试验比较7种不同大孔树脂对枳壳总黄酮的吸附性能,筛选最佳大孔树脂型号;采用单因素试验考察大孔树脂的吸附和洗脱条件。结果:AB-8型大孔树脂最适合用于纯化枳壳中总黄酮;其最佳分离、纯化工艺参数为上样液质量浓度0.408 g.L-1,pH 4,上样速率2 BV.h-1,6 BV 75%乙醇以1 BV.h-1进行洗脱;总黄酮纯度由原来的14.03%提高到42.54%。结论:优选的分离、纯化工艺稳定可行,可推广于大生产应用。
Objective： To optimize separation and purification technology of total flavonoids from Citrus aurantium by AB-8 macroporous resin. Method： Adsorption property of seven different kinds of macroporous resins for total flavonoids from C. aurantium was compared by static adsorption test, in order to select optimum macroporous resin; Single factor test was used to investigate adsorption and elution conditions of macroporous resin. Result： AB-8 macroporous resin was the most suitable one for purification of total flavonoids in C. aurantium ; Its optimum separation and purification parameters were as followings ： the sample concentration 0. 408 g L-1 , pH of 4, sample flow rate 2 BV.h-1 , eluted with 6 BV 75% ethanol at 1 BV-h-1 ; Under this condition, purity of tOtal flavonoids would be increased from 14.03% to 42.54%. Conclusion： Optimized separation and purification technology was stable and feasible, it could be extended to industrial production.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第4期14-17,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(31060210)
关键词
枳壳
总黄酮
大孔树脂
静态吸附
动态吸附
Citrus aurantium
total flavonoids
macroporous resin
static adsorption
dynamic adsorption
