摘要
目的以附子-干姜药对为研究对象,采用高效液相色谱法测定附子单煎液及附子与干姜合煎液中次乌头碱(HA)、苯甲酰乌头原碱(BAC)、苯甲酰次乌头原碱(BHA)及苯甲酰新乌头原碱(BMA)4种乌头类生物碱,探讨两药相须配伍增效原理。方法 Kromasil C18柱(4.6 mm×250 mm,5μm);流动相:A相为乙腈,B相为0.2%醋酸水溶液(三乙胺调pH为6.2),梯度洗脱(0～15 min:25%～31%A;15～36 min:31%～35%A;36～40 min:35%～25%A);流速1.0 mL·min-1,检测波长240 nm,柱温30℃。结果 4种乌头类生物碱均达到基线分离,线性关系良好(r>0.999),加样回收率为96.9%～101.9%,RSD<3%。与附子单煎液相比,附子与干姜合煎液中次乌头碱及苯甲酰新乌头原碱煎出量增加,苯甲酰乌头原碱及苯甲酰次乌头原碱煎出量稍有减少,但4种乌头类生物碱煎出量总和呈增加趋势。结论附子与干姜配伍可促进乌头类总生物碱的溶出,从成分角度解释附子与干姜相须配伍增效原理。
To explore the mechanism of compatibility of Radix Aconiti Lateralis Praeparate and Zingiber offiicinaleBosc. by determining the contents of four aconitine alkaloids （hyacinthin, benzoylaconine, benzoylhypaconine and benzoylmesaconine） in decoction made from Radix Aconiti Lateralis Praeparate alone and the co-decoctions made from the two medicinal herbs by HPLC. METHODS The separation was performed on a Kromasil C18 column（4. 6 mm × 250 mm,5 μm） with a gradient elution of acetonitrile （A） and 0. 2% aqueous acetic acid at pH of 6. 20 adjusted with triethylamine （B） （0 - 15 min ： 25 % - 31% A ; 15 - 36 rain ： 31% - 35%A;36 -40 rain：35% -25%A）. The column temperature was set at 30 ~C. The flow rate was 1.0 mL · min-1 and the injection volume was 20 μL. The detection wavelength was set at 240 nm. RESULTS The four components showed baseline separation. The linearity of this method was good （r 〉 0. 999） , and the average recoveries were 96. 9% - 101. 9% with RSD 〈 3%. Compared with the decoction made from Radix Aconiti Lateralis Praeparate alone, the contents of hyacinthin and benzoylmesaconine in the co-decoctions made from Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. were increased, the contents of benzoylaconine and benzoylhypaconine were decreased, but the sum of the four aconitine alkaloids was increased. CONCLUSION The compatibility of Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. can increase the release of aconitine alkaloids. The feasibility of the compatibility of Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. is elaborated from chemical point of view. KEY WORDS： Radix Aconiti Lateralis Praeparate; Zingiber offiicinale Bosc. ; compatibility; aconitine alkaloid; HPLC
出处
《中国药学杂志》
CAS
CSCD
北大核心
2013年第4期258-261,共4页
Chinese Pharmaceutical Journal
关键词
附子
干姜
配伍
乌头类生物碱
高效液相色谱法
Radix Aconiti Lateralis Praeparate
Zingiber offiicinale Bosc.
compatibility
aconitine alkaloid
HPLC
