摘要
以半夏块茎和顶芽为试材,对顶芽愈伤组织诱导、顶芽愈伤组织不定芽分化、块茎不定芽分化和不定芽诱导生根的最佳条件进行了研究。结果表明:半夏最佳顶芽愈伤组织诱导培养基为MS+6-BA 2.0mg/L+NAA 0.2mg/L;最佳顶芽愈伤组织不定芽分化培养基为MS+6-BA 1.0mg/L+NAA 0.2mg/L;最佳半夏块茎不定芽分化培养基为MS+6-BA 1.0mg/L+NAA 0.2mg/L;最佳不定芽诱导生根培养基为MS+2,4-D 2.0mg/L+KT 1.0mg/L。
Taking terminal bud and tuber as explants,the optimal conditions for inducing callus from terminal bud,differentiating of adventitious buds from callus,differentiating of adventitious buds from tuber and rooting of test-tube seedlings of Pinellia ternata（Thunb.） Breit.were studied.The results showed that the best medium to induce callus was MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L,MS＋6-BA 1.0 mg/L＋NAA 0.2 mg/L was the best differentiation adventitious bud medium from terminal bud callus or tuber,the optimal culture medium was MS＋2,4-D 2.0 mg/L＋KT 1.0 mg/L for rooting.
出处
《北方园艺》
CAS
北大核心
2013年第4期113-117,共5页
Northern Horticulture
关键词
半夏
组织培养
顶芽
块茎
Pinellia ternate（Thunb.） Breit.
tissue culture
terminal bud
tuber
