摘要
目的:优化苜蓿黄酮的提取富集工艺。方法:采用均匀设计法对苜蓿黄酮提取工艺进行优化,采用大孔树脂对总黄酮进行富集工艺研究。结果:最佳提取工艺为固液比为1∶19,提取2次,提取时间128min,乙醇浓度71%,提取率预测值为34.2mg/g。大孔树脂富集纯化工艺采用D101树脂,以3BV用量的70%的乙醇作为洗脱剂进行洗脱,洗脱流速为2ml/min。结论:优化的苜蓿总黄酮提取、富集工艺经济、稳定、合理可行。
Objective：To optimize the extraction technology of total flavonoids from Medicago sativa.Methods：Select the contents of total flavonoids from extract solution as the target,the conditions of extraction were optimized by the uniform design method.Results：The optimum extraction technology was as follows： solid-liqu ilratio 1∶ 19,71% ethanol,refluxing and extracting for 128min,two times.The results indicated that D101 macroporous resins had better adsorption and desorption property for purification.The best condition was determined： Best mix sample than one;the biggest was a sample weight more than twenty;the elution solvent 3 BV of 70% ethanol,the flowrate at 2ml/min.Conclusion：This optimized process is economical,stable and reasonable.
出处
《中医药信息》
2013年第1期62-65,共4页
Information on Traditional Chinese Medicine
关键词
紫花苜蓿
总黄酮
分离
均匀设计法
大孔树脂
纯化
Medicago sativa
Total flavonoids
Separation
Uniform design
Macroporous resin
Purification
