摘要
目的考察培养法与PCR法用于临床检测肺炎支原体的差异及相关药敏分析。方法用培养法和PCR法平行检测收集自河南省人民医院与郑州人民医院的433份临床呼吸道样本。结果培养法阳性率为22.86%,PCR法阳性率为24.94%,两种检测方法经χ2检验无显著性差异(P>0.05),肺炎支原体在临床对抗生素出现不同程度的耐药现象,其中大环内酯类耐药情况普遍高于其他抗生素。结论培养法检测肺炎支原体技术日趋成熟,并且与药敏检测可以同步进行,对临床肺炎支原体感染的检测和耐药性监测具有重要意义,适合各级医院的推广使用。
Objective To investigate the difference between the methods of culture and PCR in clinical applications and analyze drug susceptibility testing of Mycoplasma pneumoniae by culture and PCR. Methods A total of 433 clinical respiratory specimens were collected from the Hospital of Henan Province and Zhengzhou People＇s Hospital and tested by both methods of culture and PCR. Results The positive rate of culture method was 22.86% , and the positive rate of PCR method was 24.94%. There was no significant difference between the two detection methods by chi - square test （P 〉 0.05）. Mycoplasma pneumoniae presented varying degrees of antibiotic re- sistance in clinical detection, among which, the resistance against macrolide was generally higher than other types of antibiotics. Conclu- sion The technology of detection of Mycoplasma pneumoniae by liquid medium culture method is matureing. And it could be carried out simultaneously with the drug susceptibility testing, which is of great significance for the clinical detection of Mycoplasma pneumoniae and drug resistance surveillance. It＇s suitable for hospital at all levels.
出处
《医学研究杂志》
2013年第2期164-166,共3页
Journal of Medical Research
关键词
肺炎支原体
PCR
培养
耐药
Mycoplasma pneumoniae
PCR
Culture
Resistance
