摘要
目的研究人端粒酶逆转录酶对胶质瘤细胞系体外生物学行为的影响,以期寻找控制胶质瘤细胞恶性行为的有效途径。方法应用小分子RNA干扰技术抑制T98G胶质瘤细胞系中端粒酶逆转录酶的表达,用MTT法、TUNEL法、Transwell分别检测T98G胶质瘤细胞中人端粒酶逆转录酶被抑制后对细胞体外增殖能力、细胞凋亡、细胞侵袭、迁移能力等生物学行为的影响。结果人端粒酶逆转录酶小分子RNA干扰片段能够有效抑制酶活性,抑制T98G胶质瘤细胞增殖(P<0.05)、诱导细胞凋亡(P<0.05)并抑制细胞的迁移与侵袭(P<0.05)。结论通过抑制人端粒酶逆转录酶的活性能够有效抑制胶质瘤细胞的体外恶性生物学行为,促进细胞凋亡。
Objective To study the effects of human telomerase reverse transcriptase （hTERT） on the biological behavior of glioma cell line in vitro in order to seek an effective way of controlling malignant behavior of glioma. Methods Small molecule RNA interference technique was used to inhibit hTERT expression in T98G cell line. MTT, TUNEL and transwell methods were used to detect cell proliferation, apoptosis, invasion, migration and other biological behaviors when hTERT was inhibited in vitro. Results Small molecule RNA interference fragment could effectively inhibit hTERT activities and the proliferation of T98G glioma cells （P〈0.05）, induce apoptosis （P〈0. 05）, and inhibit cell migration and invasion （P〈O. 05）. Conclusion Inhibiting hTERT activities can effectively restrain malignant biological behavior of glioma cells in vitro, thus promoting apoptosis.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2013年第2期149-154,共6页
Journal of Xi’an Jiaotong University（Medical Sciences）
关键词
小分子RNA干扰
人端粒酶逆转录酶
增殖
凋亡
迁移
侵袭
胶质瘤
small molecule RNA interference
human telomerase reverse transcriptase （hTERT）
prolifera-tion
apoptosis
migration
invasion
glioma
