摘要
建立了高效液相色谱法(HPLC)测定猪肉与鸡肉中替米考星、泰乐菌素残留量的检测方法。样品用乙腈提取,C18固相萃取小柱净化,采用高效液相色谱分离,二极管阵列检测器(PDA)检测,外标法定量。结果表明:标准校准曲线在30～1000μg/L的范围内呈良好线性关系,相关系数r>0.999。样品中替米考星、泰乐菌素的添加水平分别为0.05、0.10、0.15mg/kg时,样品加标回收率为72.7%～90.8%,变异系数在2.6%～8.3%之间,替米考星和泰乐菌素方法检出限30μg/kg,定量限为50μg/kg。本法简便、快速、定量准确、精密度高,适用于猪肉和鸡肉中替米考星与泰乐菌素残留量的检测。
An SPE-HPLC method to determine tilmicosin and tylosin residues in pork and chicken has been established. Samples were extracted with acetonitrile and extracted antibiotic residues were cleaned up using a Ct8 SPE column, separated by HPLC, detected with a PDA detector and quantified by external standard method. The established calibration curve showed good linearity in the range of 30 to 1000 μg/L （r〉0.999）. The recovery rates of tilmicosin and tylosin from samples spiked at concentrations of 0.05, 0.10 mg/kg and 0.15 mg/kg were 72.7%-90.8%, with coefficients of variation of 2.6%-8.3%. The limits of detection （LODs） and limits of quantification （LOQs） for both antibiotics were 30 μg/kg and 50 μg/kg, respectively. The HPLC method was simple, rapid, accurate, precise and suitable to determine tilmicosin and tylosin residues in pork and chicken.
出处
《肉类研究》
2012年第12期12-15,共4页
Meat Research
关键词
高效液相色谱法
猪肉
鸡肉
替米考星
泰乐菌素
high performance liquid chromatography （HPLC）
pork
chicken
tilmicosin
tylosin
