摘要
以金合欢2 a生优树的带芽茎段为材料,着重研究不同激素浓度及其配比对金合欢组织培养各环节的影响,探索适宜的启动、增殖、生根培养基配方。结果表明:适宜的启动培养基配方为:3/4MS+6-BA1.0 mg.L-1+IBA0.5 mg.L-1;适宜的增殖培养基配方为:MS+6-BA0.5 mg.L-1+NAA0.3 mg.L-1+NaH2PO440 mg.L-1,月增殖系数可达3.83;适宜的生根培养基配方为:1/2MS+IBA0.6 mg.L-1+NAA0.2 mg.L-1,生根率可达86%,生根效果较理想。
Studies were made of medium formulas bud inducement, sub-culture and rooting culture in or- der to rapidly propagate Acaciafarnesiana. The results showed that in the medium of 3/4MS ＋ 6 - BA 1.0 mg . L-1 ＋ IBA 0. 5 mg . L-1, the optimal bud inducement was obtained, and shoots were effectively propagated in 3/4MS medium supplemented with BA 1.0 mg . L-1 , IBA 0. 5 mg. L-1 and NaH2PO4 40. 0 mg . L- 1. And for rooting, the most effective medium was half-strength MS medium supplemented with IBA 0. 6 mg . L-1 and NAA 0. 2 mg . L-1 ,and the rooting rate could reach 86%.
出处
《四川林业科技》
2013年第1期67-69,共3页
Journal of Sichuan Forestry Science and Technology
基金
四川省农作物育种攻关项目"林竹新品种选育"课题"重要经济树种优良种质资源收集
评价和育种新材料创制"专题
关键词
金合欢
组织培养
Acacia farnesiana （ L. ） Willd,Tissue culture
