摘要
目的研究探讨淫羊藿多糖对甲型H1N1流感病毒裂解疫苗的免疫佐剂活性。方法淫羊藿多糖G1和G4,生理盐水分别与流感病毒裂解疫苗配伍免疫小鼠,初次免疫后2周收集小鼠血清,ELISA法检测血清中抗体的滴度;MTT法测定脾淋巴细胞增殖反应;ELISA测定IFN-γ和IL-4的水平,流式细胞术测定脾淋巴细胞CD4+、CD8+、CD3+和CD19+亚群。结果初次免疫后,淫羊藿多糖G1和G4能显著提高免疫小鼠血清的抗体滴度;能明显增强脾T淋巴细胞的增殖反应,可以显著提高脾淋巴细胞CD4+、CD8+和CD3+的含量及CD3+/CD19+的比值,诱导分泌IFN-γ的水平升高。结论淫羊藿多糖可增强小鼠对H1N1的免疫应答,可以作为甲型H1N1流感病毒裂解疫苗的一种新型免疫佐剂。
Objective To study the adjuvant effect of Epimedium polysaccharide co-immunized with H1N1 influenza virus split vaccine.Methods BALB/c mice were intramuscularly injected H1N1 virus lysate premixed with Epimedium polysaccharide G1,G4 and saline,respectively.The titer of serum antibody of mice was determined by ELISA two weeks after the initial immunization.The proliferation of T lymphocytes was measured by MTT assay and the levels of lymphocyte cytokines IFN-γ and IL-4 were determined by ELISA.CD4^＋,CD8^＋,CD3^＋ and CD19^＋ lymphocyte populations were determined by flow cytometry.Results Epimedium polysaccharides,G1 and G4,could rapidly induce the production of high levels of serum antibody against H1N1 in mice.The polysaccharides could significantly promote lymphocytes proliferation and IFN-γ secretion.The contents of CD4^＋,CD8^＋ and CD3^＋,the ratio of CD3^＋ and CD19^＋（CD3^＋/CD19^＋） were promoted in the polysaccharides groups.Conclusion These polysaccharides could induce significant immune response against H1N1 in mice without observable toxicity.They should be further evaluated as a useful adjuvant candidate for H1N1 influenza virus split vaccine.
出处
《国际药学研究杂志》
CAS
CSCD
2013年第1期63-68,共6页
Journal of International Pharmaceutical Research
基金
国家重点基础研究发展计划资助项目(2010CB933904)
国家十二五科技支撑资助项目(2011BAD26B02)
