摘要
[目的]测定攀茎耳草总黄酮含量。[方法]以芦丁为对照品,用超声辅助提取法提取攀茎耳草中的总黄酮,再用紫外分光光度法测定总黄酮含量。[结果]在0.011～0.110 mg/ml范围内芦丁浓度与吸光度有良好的线性关系,回归方程为Y=18.015X+0.020,相关系数R2=0.999 4;攀茎耳草中总黄酮的相对百分含量为1.609%,精密度试验RSD为1.10%。[结论]该方法操作简便、结果可靠,能为攀茎耳草药用价值的开发及扩大资源利用提供科学的依据。
[ Objective] To detect the content of total flavonoids from Hedyotis scandens Roxb. [ Method ] With rutin as the reference sub- stance, total flavonoids were extracted from H. scandens by ultrasonic-assisted extraction. Then, the content of total flavonoids was detected by UV Spectrophotometry. [ Result] Rutin concentration and absorbancy had good linear relationship between 0.011 and 0.110 mg/mt. The re- gression equation was Y = 18.015X ＋ 0. 020 ; and correlation coefficient was R2 = 0. 999 4. The relative percentage of total flavonoids in H. scandens was 1. 609%. And the RSD in precision test was 1.10%. [ Conclusion] This method was simple, reliable and could provide scientific references for the develooment and utilization of the medicinal value of H. scandens.
出处
《安徽农业科学》
CAS
2013年第3期1032-1033,1046,共3页
Journal of Anhui Agricultural Sciences
基金
国家社会科学基金(12XMZ033)
贵州省科学技术基金(黔科合J字LKM[2011]06号)
