摘要
旨在研究由超导磁体产生的强磁场重力环境(HMGE)对Pseudomonas aeruginosa N1207的影响。该磁体是经特殊设计的大梯度超导磁体,可产生多个重力梯度(从失重到超重)以及相应的磁场强度,分别为(0 g,12 T)、(1 g,16 T)和(2 g,12 T)。Pseudomonas aeruginosa N1207在HMGE中分别诱变24h、48h和72h之后,从磁场强度最高(16 T)组别中筛选得到最佳的突变株M14808。结果表明M14808的鼠李糖脂产量提高了30%以上并且遗传特性稳定。比较生长周期发现,突变株到达指数生长期早于原始菌株。诱变实验结果表明强磁场是引起诱变的主要因素从而导致鼠李糖脂产量变化和生长周期改变。抗肿瘤实验结果显示双鼠李糖脂可抑制四株肿瘤细胞,分别为MCF-7、H460、HepG2和A549,双鼠李糖脂对MCF-7的效果最佳,其IC50为125.13μg/ml。
The aim is to investigate the effects of a High Magneto-Gravitational Environment （HMGE） produced by a superconducting magnet on Pseudomonas aeruginosa N1207. A specially designed large gradient superconducting magnet was used to produce gravity levels from hypo-gravity （0 g） to hyper-gravity （2 g） and relevant magnetic field strengths, namely （0 g, 12 T）, （1 g, 16 T） and （2 g, 12 T）. After Pseudomonas aeruginosa N1207 was mutated in HMGE for 24h, 48h, 72h, respectively, the mutant M14808 with the highest yield of rhamnolipids was isolated from the strongest magnetic field （ 16 T）. Results showed that the rhamnolipid production of M14808 was dramatically increased by over 30%, and maintained a constant yield for 6 generations. Comparing the growth cycle of the original strain and mutants, it was observed that mutants reached the exponential phase earlier than the original strain. The mutation result indicated that the high magnetic field was primarily responsible for the production of rhamnolipids and the growth rate. The antitumor cytotoxicity experiment showed that crude di-rhamnolipids could inhibit the growing of four cancer cell lines, MCF-7, H460, HepG2 and A549, with the ICon of di-rhamnolipids to MCF-7 as low as 125.13μg/ml.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2013年第2期27-33,共7页
China Biotechnology
基金
supported by the National"863"High Technologies Research Foundation of China(No.2088AA12A218-12)~~
关键词
强磁场重力环境
鼠李糖环脂诱变育种铜绿假单胞菌
生长周期抗肿瘤活性
High magneto-gravitational environment Rhamnolipids Mutation breeding Pseudomonasaeruginosa Growth cycle Antitumor activity
