摘要
目的探讨丹参注射剂对高氧诱导下的早产儿视网膜病变小鼠视网膜新生血管（Retinal neovascularization，RNV）的抑制作用。方法采用随机对照原则，将出生7日龄的C57BL／6J小鼠54只分为A（单纯对照组）、B（高氧诱导模型组）、C（丹参治疗组）3组，每组18只。B组与c组小鼠在氧气浓度为75％的环境中饲养5日，然后回到正常氧环境中与A组一同饲养。这三组小鼠分别在第12、14、17天各随机处死6只。分别采用Isolectin-B4（GREEN）染色法进行视网膜铺片术观察视网膜新生血管的形态，视网膜组织切片HE染色，观察计算突破视网膜内界膜的血管内皮细胞核的数量，同时以免疫组织化学染色的方法检测视网膜组织切片各层中血管内皮生长因子（Vascular endothelial growth factor，VEGF）的表达。结果Isolectin-B4（GREEN）染色法视网膜铺片中，B组视网膜可以见到大量的新生血管形成，荧光渗漏明显，C组较之新生血管明显减少，荧光渗漏轻，A组未见新生血管和荧光渗漏；在突破视网膜内界膜的血管内皮细胞核计数中见到，A组〈c组〈B组，差异具有统计学意义（P〈O．05）；VEGF的表达在B组的视网膜各层均很明显，C组则主要在靠近视网膜内层的部分比较明显，但较B组明显减弱，VEGF在A组的视网膜中表达不明显。结论丹参注射液对视网膜VEGF的表达有明显抑制作用，可减少早产儿视网膜病变中新生血管的形成。
Objective To investigate the suppressing effect of salvia miltiorrhiza on retinal neovascularization （RNV） in rats. Methods According to random control principle, 54 seven-day-old rats were divided into three groups, including A （control group）, B （hyperoxia group） and C （salvia milriorrhiza group）, 18 in each group. To establish an oxygeninduced retinopathy, hyperoxia group and salvia milriorrhiza group were exposed to 75% oxygen continuously for five days. Salvia miltiorrhiza group received intraperitoneal injections of salvia miltiorrhiza every day since the rats were born, and the injection would continue for the following five days after moving out of the hyperox- ia circumstance. The three groups of rats were randomly executed 6 respectively on 12th, 14th and 17th day. Isolectin-B4 （GREEN） staining was used to observe the retina vascular morphology respectively. HE staining following retina tissue cross-sections was used to calculate the nuclei of neovascularization extending from inner limiting membrane into vitreous in tissue slice. Expression levels of VEGF protein was measured by immunohistoehemical method. Results Isolectin-B4 （GREEN） stain- ing showed that a great deal of neovascularization with obvious fluorescein leakage formed in the group B, while obviously less neovascularization and less fluorescein leakage in group C and no neovascularization and fluorescein leakage was presented in group A. The nuclei counting indicated that vascular endothelial cell number significantly increased in group B compared with group C （P 〈 0.05）, while there was almost no cells extending from the inner limiting membrane in group A. The expression of VEGF was obvious in various layers in group B, while group C was relatively obvious in the layers near the inner part of retina. Conclusion Salvia miltiorrhiza can obviously inhibit the expression of VEGF, and particularly decrease the retinal neovascularization caused by hyperoxia circumstance in rats.
出处
《中国实用眼科杂志》
CSCD
北大核心
2013年第2期234-238,共5页
Chinese Journal of Practical Ophthalmology
基金
广东省中医药局2010年建设中医药强省科研课题（2010363）
关键词
丹参
视网膜新生血管
血管内皮生长因子
Salvia miltiorrhiza
Retinal neovascularization
Vascular endothelial growth factor
