摘要
以吡咯伯克霍尔德氏菌Burkholderia pyrrocinia JK-SH007为出发菌株,对其发酵工艺进行优化,以期提高发酵效率。通过筛选试验、最陡爬坡试验和响应面分析确定影响JK-SH007菌株生长最重要两因素为玉米浆和葡萄糖,其最佳浓度分别为13.88 g/L和3.37 g/L。优化后的发酵工艺培养该菌浓度可达1.18×109CFU/mL,比优化前提高1.35倍,抑菌活性提高28.84%。
In order to improve ferment efficiency of biocontrol agent Burkholderia pyrrocinia JK-SH007, the fermentation conditions of this strain were optimized. The optimal fermentation conditions were corn steep liquor (13.88 g/L) and glucose (3.37 g/L) by screening test, steepest ascent experiments and response surface analysis. The results showed that the cell density of JK-SH007 (1.18×10^9 CFU/mL) increased 1.35 times than before, and there was a 28.84% increase in antifungal activity.
出处
《生物工程学报》
CAS
CSCD
北大核心
2013年第2期243-246,共4页
Chinese Journal of Biotechnology
基金
国家博士后基金(No.20110491434)
国家林业公益性行业科研专项项目(No.201304404)
江苏省普通高校研究生科研创新计划(No.CXZZ11_0505)资助~~
关键词
吡咯伯克霍尔德氏菌
发酵
响应面法
玉米浆
生防菌
Burkholderia pyrrocinia, fermentation, response surface methodology, corn steep liquor, biocontrol agent