摘要
目的研究二甲基甲酰胺（DMF）暴露工人的血清ALT、外周血DNA损伤及血浆谷胱甘肽（GsH）含量，并分析其与暴露水平之间的关系。方法选择50名DMF作业工人为暴露组，同一地区不接触DMF的50名工人为对照组，测定暴露工人班末尿中甲基甲酰胺（NMF）的含量，并检测血清ALT含量及血浆GSH含量，用彗星试验检测外周血DNA损伤。结果暴露组ALT、DNA损伤分别为（29．66±21．76）U／L和5．36±3．33，均显著高于对照组（t=3．067、4．512，P〈0．01），暴露组GSH含量为（6．38±1．70）μmol／L，显著低于对照组（t=-6．811，P〈0．01）。对照组、低暴露组（尿NMF0—10mg／L）和高暴露组（尿NMF〉10mg／L）的ALT含量，DNA损伤及GSH含量差异均有统计学意义（F=5．46、12．27、23．42，P〈0．01），两两比较发现低暴露组和高暴露组ALT、DNA损伤均显著高于对照组（P〈0．05，P〈0．01），而GSH含量则显著低于对照组（P〈0．01），低、高暴露组三个指标之间差异均无统计学意义（P均〉0．05）。工人尿中NMF含量与ALT、DNA损伤及GSH含量之间存在显著相关性（r=0．321、0．449、-0．544，P均〈0．01）。结论DMF可引起作业工人的肝功能损伤及外周血DNA损伤，可能和GSH含量降低有关。
Objective To analyze serum ALT, DNA damage of peripheral blood cells and glutathione（GSH） level in plasma from dimethyformamide （DMF） exposed workers and to investigate the relationship between these parameters and the exposure levels of DMF. Methods There were 50 DMF exposed workers and 50 non-DMF exposed workers enrolled in the study. The levels of N-methylformamide （NMF） in end-shift urine samples were detected. The contents of ALT in serum and GSH in plasma were measured. DNA damage in peripheral blood cells was detected by comet assay. Results The levels of ALT and DNA damage in exposed group were （29.66 ±21.76） U/L and 5.36 ± 3.33, and were significantly higher than those in the control group（t = 3.067, 4.512, P 〈 0.01）. The GSH content in exposed group was （6.38 ± 1.70） μmol/L, and was lower than that in the control （t = -6.811, P 〈 0.01 ）. The exposed group was divided into low-dose （NMF in urine： 0 - 10 nag/L） and high-dose （NMF in urine 〉 10 nag/L） groups based on the average level of NMF in urine. There were significant differences of the levels of ALT, DNA damage and GSH among control group, low-dose group and high-dose group （ F = 5.46, 12.27, 23.42, P 〈 0.01）. The levels of ALT and DNA damage in each DMF exposed group were still higher than the control （P 〈 0.05, P 〈 0.01）, and the GSH content showed a reversed result （P 〈 0.01）. The three parameters showed no significant difference between low-dose and high- dose groups. There were good correlations between the level of NMF and the levels of ALT, DNA damage, GSH, respectively （r = 0. 321, 0.449, -0.544, P 〈 0.01 ）. Conclusions DMF may result in liver damage and DNA damage to humans, which might be related to decreased GSH content in plasma.
出处
《国际流行病学传染病学杂志》
CAS
2013年第1期13-16,共4页
International Journal of Epidemiology and Infectious Disease
基金
浙江省医学支撑学科建设项目（11-ZC02）
